Davis M Wayne, Jorgensen Erik M
Howard Hughes Medical Institute and School of Biological Sciences, University of Utah, Salt Lake City, UT, United States.
Front Bioinform. 2022 Feb 4;2:818619. doi: 10.3389/fbinf.2022.818619. eCollection 2022.
A plasmid Editor (ApE) is a free, multi-platform application for visualizing, designing, and presenting biologically relevant DNA sequences. ApE provides a flexible framework for annotating a sequence manually or using a user-defined library of features. ApE can be used in designing plasmids and other constructs simulation of cloning methods such as PCR, Gibson assembly, restriction-ligation assembly and Golden Gate assembly. In addition, ApE provides a platform for creating visually appealing linear and circular plasmid maps. It is available for Mac, PC, and Linux-based platforms and can be downloaded at https://jorgensen.biology.utah.edu/wayned/ape/.
质粒编辑器(ApE)是一款免费的多平台应用程序,用于可视化、设计和展示生物学相关的DNA序列。ApE提供了一个灵活的框架,可用于手动注释序列或使用用户定义的特征库。ApE可用于设计质粒和其他构建体,模拟PCR、吉布森组装、限制性连接组装和金门组装等克隆方法。此外,ApE还提供了一个平台,用于创建视觉上吸引人的线性和环形质粒图谱。它适用于基于Mac、PC和Linux的平台,可在https://jorgensen.biology.utah.edu/wayned/ape/ 下载。