使用 ApE 进行计算机模拟 Golden Gate 克隆。

Using ApE for In Silico Golden Gate Cloning.

机构信息

School of Biological Sciences, HHMI, University of Utah, Salt Lake City, UT, USA.

出版信息

Methods Mol Biol. 2025;2850:79-87. doi: 10.1007/978-1-0716-4220-7_5.

Abstract

Golden Gate cloning allows rapid and reliable assembly of multiple DNA fragments in a defined orientation. Golden Gate cloning requires careful design of the restriction fragment overhangs to minimize undesired products and to generate the desired junctions. The ApE (A plasmid Editor) software package can assist in silico design of input fragments or to generate expected assembly products.

摘要

金门克隆技术允许以确定的方向快速可靠地组装多个 DNA 片段。金门克隆技术需要仔细设计限制酶切片段的突出端，以最大限度地减少非预期产物并生成所需的连接。ApE（质粒编辑器）软件包可用于输入片段的计算机辅助设计或生成预期的组装产物。