Gu Mingkun, Nguyen Huong Thi, Cho Joo-Hyung, Suh Joo-Won, Cheng Jinhua
Interdisciplinary Program of Biomodulation, Myongji University, Yongin, Republic of Korea.
Myongji Bioefficacy Research Center, Myongji University, Yongin, Republic of Korea.
Mol Oral Microbiol. 2023 Apr;38(2):145-157. doi: 10.1111/omi.12397. Epub 2022 Nov 14.
Lactic acid bacteria have been widely used as probiotics for improving gut health. However, studies on oral probiotics were very limited. In this study, 67 lactic acid bacteria (LAB) were isolated from fermented food and screened for antagonistic activity against Streptococcus mutans, the causative pathogen of dental caries. Leuconostoc mesenteroides MJM60376 showed the highest antagonistic activity against S. mutans KCTC3065. L. mesenteroides MJM60376 also showed oral probiotic characteristics including weak acid production, lysozyme tolerance, adhesion to oral epithelial cell (YD-38), antibiotic susceptibility, and good coaggregation ability with S. mutans. Furthermore, the biofilm formation of S. mutans was significantly reduced when cocultured with L. mesenteroides. Scanning electron microscopy analysis showed that amounts of attached bacteria of S. mutans and network-like structures were significantly reduced by L. mesenteroides MJM60376. Cell-free supernatant (CFS) of L. mesenteroides MJM60376 also greatly inhibited biofilm formation of S. mutans from the adherent stage, the activity remained even after it was treated with catalase, trypsin, or pH neutralized. Expression levels of biofilm formation-related genes were significantly reduced in S. mutans when it was treated with the CFS of L. mesenteroides MJM60376. Therefore, L. mesenteroides MJM60376 has great potential to be used as a multifunctional ingredient.
乳酸菌已被广泛用作益生菌以改善肠道健康。然而，关于口服益生菌的研究非常有限。在本研究中，从发酵食品中分离出67株乳酸菌（LAB），并筛选其对变形链球菌（龋齿的致病病原体）的拮抗活性。肠系膜明串珠菌MJM60376对变形链球菌KCTC3065表现出最高的拮抗活性。肠系膜明串珠菌MJM60376还表现出口服益生菌特性，包括产弱酸、溶菌酶耐受性、对口腔上皮细胞（YD - 38）的粘附、抗生素敏感性以及与变形链球菌良好的共聚能力。此外，与肠系膜明串珠菌共培养时，变形链球菌的生物膜形成显著减少。扫描电子显微镜分析表明，肠系膜明串珠菌MJM60376显著减少了变形链球菌的附着细菌数量和网络状结构。肠系膜明串珠菌MJM60376的无细胞上清液（CFS）也从粘附阶段极大地抑制了变形链球菌的生物膜形成，在用过氧化氢酶、胰蛋白酶处理或pH中和后，该活性仍然存在。用肠系膜明串珠菌MJM60376的CFS处理变形链球菌时，其生物膜形成相关基因的表达水平显著降低。因此，肠系膜明串珠菌MJM60376具有作为多功能成分使用的巨大潜力。
