Muharam Raden, Bustami Arleni, Gusti Mansur Indra, Zulkifli Jacoeb Teuku, Giustiniani Jerome, Schiavon Valerie, Bensussan Armand
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Master's Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Int J Reprod Biomed. 2022 Sep 6;20(8):691-700. doi: 10.18502/ijrm.v20i8.11758. eCollection 2022 Aug.
Endometriosis is believed to be associated with dysfunction of the lymphocyte population and cytotoxicity of natural killer (NK) cells, induced by the production of interleukin-2 (IL-2).
This study aimed to investigate T lymphocytes and NK cell activity in the peripheral blood mononuclear cells (PBMCs) of women with endometriosis.
PBMCs were obtained from the peripheral venous blood samples of 14 women with and without endometriosis (n = 7 for each group). Then, the PBMCs were co-cultured for 4 days and were treated with recombinant IL-2 for cytotoxic activity toward target cells (Daudi and K562 cells). The cytotoxicity activity was determined using the 51 chromium release assay before and after stimulation. Flow cytometry measurement was used to examine the expression of T lymphocytes and NK cells before and after being treated with IL-2.
The concentration of CD3+CD28+ (co-stimulatory) was significantly lower in the endometriosis group (65.62 5.38) compared to in its counterpart (50.24 4.22) (p = 0.04) before stimulation. However, no significant differences were observed in any other T lymphocytes and NK cells. It was also found that there was a significant increase of CD3-CD28+ after treatment with IL-2 only in the healthy control but not in women with endometriosis.
Increased expression of CD160 and decreased CD28 play a role in inhibiting NK cell activation and T cell response in women with endometriosis.
子宫内膜异位症被认为与淋巴细胞群功能障碍以及由白细胞介素 - 2（IL - 2）产生所诱导的自然杀伤（NK）细胞的细胞毒性有关。
本研究旨在调查子宫内膜异位症女性外周血单个核细胞（PBMCs）中的T淋巴细胞和NK细胞活性。
从14名有或无子宫内膜异位症的女性（每组n = 7）的外周静脉血样本中获取PBMCs。然后，将PBMCs共培养4天，并用重组IL - 2处理以检测其对靶细胞（Daudi和K562细胞）的细胞毒性活性。在刺激前后使用51铬释放试验测定细胞毒性活性。使用流式细胞术测量来检测在用IL - 2处理前后T淋巴细胞和NK细胞的表达。
刺激前，子宫内膜异位症组中CD3 + CD28 +（共刺激）的浓度（65.62±5.38）显著低于对照组（50.24±4.22）（p = 0.04）。然而，在任何其他T淋巴细胞和NK细胞中未观察到显著差异。还发现仅在健康对照组中用IL - 2处理后CD3 - CD28 +有显著增加，而子宫内膜异位症女性中没有。
CD160表达增加和CD28减少在抑制子宫内膜异位症女性的NK细胞活化和T细胞反应中起作用。
