Prince D L, Kotin R M, Dubin D T
Mol Biol Rep. 1986;11(1):51-5. doi: 10.1007/BF00417596.
A novel RNA fraction, 'Cy RNA,' that accumulates in mitochondria when hamster cells are treated with the methylation inhibitor cycloleucine, has been characterized by high resolution acrylamide gel electrophoresis and DNA-RNA hybridization. Cy RNA ran in gels as a discrete band, with an apparent chain length of 2 600. It hybridized specifically to restriction fragments containing genes for the mitochondrial ribosomal RNAs. We infer that Cy RNA is a discrete polycistronic ribosomal RNA precursor transcript, whose processing is dependent on normal methylation.
一种新的RNA组分“Cy RNA”，当仓鼠细胞用甲基化抑制剂环亮氨酸处理时会在线粒体中积累，已通过高分辨率丙烯酰胺凝胶电泳和DNA-RNA杂交进行了表征。Cy RNA在凝胶中呈离散条带迁移，表观链长为2600。它与包含线粒体核糖体RNA基因的限制性片段特异性杂交。我们推断Cy RNA是一种离散的多顺反子核糖体RNA前体转录本，其加工依赖于正常甲基化。
