婴儿双歧杆菌亚种来源的膜泡对IgA产生的增强作用。

Enhancement of IgA production by membrane vesicles derived from Bifidobacterium longum subsp. infantis.

作者信息

Kurata Atsushi, Yamasaki-Yashiki Shino, Imai Tomoya, Miyazaki Ayano, Watanabe Keito, Uegaki Koichi

机构信息

Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara, Japan.

Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka, Japan.

出版信息

Biosci Biotechnol Biochem. 2022 Dec 21;87(1):119-128. doi: 10.1093/bbb/zbac172.

DOI:10.1093/bbb/zbac172

PMID:36331264

Abstract

Immunoglobulin A (IgA) is involved in the maintenance of gut homeostasis. Although the oral administration of bifidobacteria increases the amount of fecal IgA, the effects of bifidobacteria on intestinal immunity remain unclear. We found and characterized membrane vesicles (MVs) derived from Bifidobacterium longum subsp. infantis toward host immune cells. Bifidobacterium infantis MVs consisted of a cytoplasmic membrane, and extracellular solute-binding protein (ESBP) was specifically detected. In the presence of B. infantis MVs or recombinant ESBP, RAW264 cells produced the pro-inflammatory cytokine IL-6. IgA was produced by Peyer's patches cells following the addition of B. infantis MVs. Therefore, ESBP of B. infantis MVs is involved in the production of IgA by acquired immune cells via the production of IL-6 by innate immune cells.

摘要

免疫球蛋白A（IgA）参与肠道内环境稳态的维持。尽管口服双歧杆菌可增加粪便中IgA的含量，但双歧杆菌对肠道免疫的影响仍不清楚。我们发现并鉴定了源自婴儿双歧杆菌亚种的膜囊泡（MVs）对宿主免疫细胞的作用。婴儿双歧杆菌MVs由细胞质膜组成，并特异性检测到细胞外溶质结合蛋白（ESBP）。在存在婴儿双歧杆菌MVs或重组ESBP的情况下，RAW264细胞产生促炎细胞因子IL-6。添加婴儿双歧杆菌MVs后，派尔集合淋巴结细胞产生IgA。因此，婴儿双歧杆菌MVs的ESBP通过天然免疫细胞产生IL-6参与获得性免疫细胞产生IgA的过程。