Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing, 100083, China.
College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
Biosens Bioelectron. 2023 Jan 15;220:114844. doi: 10.1016/j.bios.2022.114844. Epub 2022 Oct 29.
A novel colorimetric biosensor was explored for fast, sensitive, and on-site detection of Salmonella on a microfluidic chip employing immune gold@platinum nanoparticles (Au@Pt NPs) for specific bacterial labeling, a finger-driven mixer with two serial air chambers for efficient immunoreaction and a nuclear track membrane as specific-size microfilter for effective bacterial isolation from excessive immune Au@Pt NPs. First, the bacterial sample and immune Au@Pt NPs were pipetted into the mixing chamber and mixed sufficiently through repeated press-release actions on the small air chamber which could precisely control the air volume, leading to the formation of bacteria-immune Au@Pt NP conjugates. Then, the small and large air chambers were pressed simultaneously to push all the solution in the mixing chamber to flow through the microfilter for trap of the formed larger-size conjugates on the membrane and removal of the unbound smaller-size immune Au@Pt NPs. After the HO-TMB substrate was pipetted into the microfilter and catalyzed by the conjugates, ImageJ was used to analyze the color change for bacterial determination. This simple biosensor enabled Salmonella detection as low as 168 CFU/mL in 25 min.
一种新型比色生物传感器被探索用于在微流控芯片上快速、灵敏和现场检测沙门氏菌，该传感器采用免疫金@铂纳米粒子（Au@Pt NPs）用于特定细菌标记、手指驱动混合器，带有两个串联的气室，用于有效免疫反应和核径迹膜作为特定尺寸的微滤器，用于从过量的免疫 Au@Pt NPs 中有效分离细菌。首先，将细菌样品和免疫 Au@Pt NPs 吸入混合室，并通过在小气室上反复进行按压-释放操作充分混合，该操作可以精确控制空气量，从而形成细菌-免疫 Au@Pt NP 缀合物。然后，同时按压小气室和大气室，将混合室中的所有溶液推过微滤器，以捕获形成的较大尺寸缀合物并去除未结合的较小尺寸免疫 Au@Pt NPs。将 HO-TMB 底物吸入微滤器并被缀合物催化后，使用 ImageJ 分析颜色变化以进行细菌测定。这种简单的生物传感器能够在 25 分钟内检测到低至 168 CFU/mL 的沙门氏菌。
