College of Pharmaceutical Sciences, Guizhou University of Traditional Chinese Meidicine, Guiyang 550025, China.
Nano-Drug Technology Research Center, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China.
Molecules. 2023 Aug 5;28(15):5889. doi: 10.3390/molecules28155889.
Nanoemulsion is a new multi-component drug delivery system; the selection of different oil phases can give it special physiological activity, and play the role of "medicine and pharmaceutical excipients all-in-one". In this paper, we used glycyrrhizic acid as the natural surfactant, and oil (BB) and tea tree oil (TTO) as the mixed oil phase, to obtain a new green functional composite nanoemulsion. Using the average particle size and polydispersion index (PDI) as the evaluation criteria, the effects of the oil ratio, oil content, glycyrrhizic acid concentration, and ultrasonic time on the nanoemulsion were systematically investigated. The stability and physicochemical properties and biological activities of BB-TTO NEs prepared via the optimum formulation were characterized. The optimal prescription was BB: TTO = 1:1, 5% oil phase, 0.7% glycyrrhizic acid, and 5 min ultrasonication time. The mean particle size, PDI, and zeta potential were 160.01 nm, 0.125, and -50.94 mV, respectively. The nanoemulsion showed non-significant changes in stability after centrifugation, dilution, and 120 days storage. These nanoemulsions were found to exhibit potential antibacterial and anti-inflammatory activities. The minimal inhibitory concentration (MIC) of BB-TTO NEs against , , and is 2975 μg/mL, 2975 μg/mL, and 5950 μg/mL, respectively. A lower level of inflammatory cell infiltration and proportion of fibrosis were found in the synovial tissue of AIA rats treated with BB-TTO NEs. These findings demonstrate that the BB-TTO NEs produced in this study have significant potential for usage in antibacterial and anti-inflammatory areas.
纳米乳是一种新型的多组分药物传递系统；选择不同的油相可以赋予其特殊的生理活性，并发挥“药辅一体”的作用。本研究以甘草酸为天然表面活性剂，以蓖麻油（BB）和茶树油（TTO）为混合油相，制备了一种新型的绿色功能性复合纳米乳。以平均粒径和多分散指数（PDI）为评价指标，系统考察了油相比例、油含量、甘草酸浓度和超声时间对纳米乳的影响。对最佳处方制备的 BB-TTO NEs 的稳定性、理化性质和生物活性进行了表征。最佳处方为 BB：TTO=1：1，油相 5%，甘草酸 0.7%，超声 5 min。所得纳米乳的平均粒径、PDI 和 Zeta 电位分别为 160.01nm、0.125 和-50.94mV。纳米乳经离心、稀释和 120 天贮存后稳定性无显著变化。该纳米乳具有潜在的抗菌和抗炎活性。BB-TTO NEs 对 、 、 的最小抑菌浓度（MIC）分别为 2975μg/mL、2975μg/mL 和 5950μg/mL。BB-TTO NEs 处理 AIA 大鼠的滑膜组织中炎性细胞浸润和纤维化比例较低。这些结果表明，本研究制备的 BB-TTO NEs 在抗菌和抗炎领域具有很大的应用潜力。
