Ashagrie Yenework Nigussie, Tadesse Mesfin Getachew, Bachheti Rakesh Kumar, Nijhawan Ginni, Tyagi Sheetal, Bachheti Archana
Nanotechnology Center of Excellence, Addis Ababa Science and Technology University, P.O. Box 16417, Addis Ababa, Ethiopia.
Department of Industrial Chemistry, College of Natural and Applied Science, Addis Ababa Science and Technology University, P.O. Box 16417, Addis Ababa, Ethiopia.
Sci Rep. 2025 Apr 26;15(1):14598. doi: 10.1038/s41598-025-87732-y.
Nanoemulsions contain acceptable O/W or W/O dispersions, with droplet sizes ranging from 100 to 500 nm. In the current study, we formulated O/W nanoemulsion using Caesalpinia decapetala seed oil, Tween 20, and Tween 80 surfactant through ultrasonic and spontaneous emulsification. C. decapetala is a climbing shrub thorny tree. C. decapetala seed oil contains different chemical constituents. As a result, physicochemical properties of prepared nanoemulsion came to be 132.561 ± 0.491-290.033 ± 1.952 nm average particle size, 0.028 ± 0.038-0.301 ± 0.042 polydispersive index, -32.274 to -58 mV zeta potentials, 4.383-6.5 pH value, 3.922-5.247 mPa.s viscosity value with spherical shape, with excellent physical stability. Subsequently, gram-positive and gram-negative bacterial strains were employed to assess the nanoemulsion's antibacterial efficacy. Therefore, the results indicate that C. decapetala seed oil nanoemulsion has excellent antibacterial activity on gram-positive and gram-negative bacteria strains. This nanoemulsion formulated using Tween 80 had higher inhibition zones like 13.5,13, 12, and 11 mm than the other methods on S. aureus, E.coli, S.pyogenes, and P. aeruginosa, respectively. Generally, smaller particle size, polydispersive index, stable surface charge, and low value of viscosity indicate that formulated nanoemulsion had better stability and bioavailability activity for antibacterial activity.
纳米乳剂包含可接受的水包油（O/W）或油包水（W/O）分散体，液滴尺寸范围为100至500纳米。在本研究中，我们通过超声和自发乳化，使用云实籽油、吐温20和吐温80表面活性剂制备了水包油纳米乳剂。云实是一种攀援状有刺灌木。云实籽油含有不同的化学成分。结果，所制备纳米乳剂的物理化学性质为平均粒径132.561±0.491 - 290.033±1.952纳米，多分散指数0.028±0.038 - 0.301±0.042，ζ电位-32.274至-58毫伏，pH值4.383 - 6.5，粘度值3.922 - 5.247毫帕·秒，呈球形，具有优异的物理稳定性。随后，使用革兰氏阳性和革兰氏阴性细菌菌株评估纳米乳剂的抗菌效果。因此，结果表明云实籽油纳米乳剂对革兰氏阳性和革兰氏阴性细菌菌株具有优异的抗菌活性。这种使用吐温80制备的纳米乳剂对金黄色葡萄球菌、大肠杆菌、化脓性链球菌和铜绿假单胞菌的抑菌圈分别为13.5、13、12和11毫米，比其他方法更高。一般来说，较小的粒径、多分散指数、稳定的表面电荷和较低的粘度值表明所制备的纳米乳剂具有更好的稳定性和抗菌活性生物利用度。
