Oktanella Yudit, Mustofa Imam, An-Haru Fahrunnisak Al-Firda Razak, Putri Desinta Dwi Melati, Hendrawan Viski Fitri, Susilowati Suherni, Degu Nurhusien Yimer, Hernawati Tatik
Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Brawijaya University, Malang, East Java, Indonesia.
Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Airlangga University, Jl. Dr. Ir. H. Soekarno, Mulyorejo, Kec. Mulyorejo, Surabaya, East Java, Indonesia.
Vet World. 2024 Jul;17(7):1637-1647. doi: 10.14202/vetworld.2024.1637-1647. Epub 2024 Jul 30.
The use of frozen goat semen for artificial insemination frequently results in a decline in sperm quality following thawing, which can be attributed to cold shock from cryopreservation, reduced motility, and possible DNA damage. Freezing may compromise mRNA stability due to the presence of free radicals. Despite strong post-thaw motility and no visible DNA fragmentation, sperm can still exhibit altered gene expression patterns. To reduce the damaging impact of free radicals during cryopreservation, antioxidants are typically added to the freezing medium. This study assessed the impact of adding coenzyme Q10 (CoQ10) to frozen sperm diluent on the ATP5F1A and CPT2 gene expression, sperm motility, and viability post-thawing.
CoQ10 was added to sperm at six different concentrations: 0 mg/dL (P0), 6.25 mg/dL (P1), 12.5 mg/dL (P2), 25 mg/dL (P3), 50 mg/dL (P4), and 100 mg/dL (P5). The Statistical Package for the Social Sciences (SPSS) software version 22 was used to conduct comparative tests using one-way analysis of variance followed by Duncan's test for motility and viability and Kruskal-Wallis test followed by pairwise comparison test for membrane integrity and gene expression.
The addition of CoQ10 to semen diluent has a notable impact on the post-thawed quality of sperm. The most significant outcomes were observed with a 25 mg/dL dosage (P3) for cell viability, membrane integrity, and ATP5F1A gene expression, and with a 50 mg/dL dosage (P4) for sperm motility, membrane integrity, and CPT2 gene expression.
Incorporating CoQ10 into frozen semen diluent improves gene expression and prevents deterioration of the cell quality of thawed goat spermatozoa. While the study demonstrates the benefits of CoQ10, the precise molecular mechanisms through which CoQ10 enhances gene expression and cell quality were not fully elucidated. Further investigation is needed to understand these mechanisms in detail. Comparative studies with other antioxidants and cryoprotectants can help establish the relative efficacy of CoQ10 and potentially develop more effective combinations.
使用冷冻山羊精液进行人工授精时，解冻后精子质量常常下降，这可能归因于冷冻保存引起的冷休克、活力降低以及可能的DNA损伤。由于自由基的存在，冷冻可能会损害mRNA的稳定性。尽管解冻后精子活力强且无可见的DNA片段化，但精子仍可能表现出基因表达模式的改变。为了减少冷冻保存过程中自由基的破坏作用，通常会在冷冻培养基中添加抗氧化剂。本研究评估了在冷冻精子稀释液中添加辅酶Q10（CoQ10）对ATP5F1A和CPT2基因表达、精子活力及解冻后存活率的影响。
将CoQ10以六种不同浓度添加到精子中：0mg/dL（P0）、6.25mg/dL（P1）、12.5mg/dL（P2）、25mg/dL（P3）、50mg/dL（P4）和100mg/dL（P5）。使用社会科学统计软件包（SPSS）22版进行比较测试，对活力和存活率采用单因素方差分析后进行邓肯检验，对膜完整性和基因表达采用克鲁斯卡尔-沃利斯检验后进行两两比较检验。
在精液稀释液中添加CoQ10对解冻后精子质量有显著影响。对于细胞活力、膜完整性和ATP5F1A基因表达，在25mg/dL剂量（P3）时观察到最显著的结果；对于精子活力、膜完整性和CPT2基因表达，在50mg/dL剂量（P4）时观察到最显著的结果。
将CoQ10加入冷冻精液稀释液可改善基因表达并防止解冻后山羊精子细胞质量的恶化。虽然该研究证明了CoQ10的益处，但CoQ10增强基因表达和细胞质量的确切分子机制尚未完全阐明。需要进一步研究以详细了解这些机制。与其他抗氧化剂和冷冻保护剂的比较研究有助于确定CoQ10的相对功效，并可能开发出更有效的组合。
