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转座子插入对……营养生长过程中转录的动态影响

The Dynamic Effects of Transposon Insertions on Transcription During Vegetative Development in .

作者信息

Troy Julia M, Bhalla Disha, Cadet Kayla M, Dishmey Angeline G, Hernandez Nathaly M, Ishraq Zuhair, Marchand Chloé, Markoulis Spyro, Nelson Resa B, Xhindolli Nicholas, Wang Dafang F

机构信息

Biology, Hofstra University, Hempstead, New York, United States.

出版信息

MicroPubl Biol. 2025 Jul 14;2025. doi: 10.17912/micropub.biology.001640. eCollection 2025.

DOI:10.17912/micropub.biology.001640
PMID:40735503
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12305366/
Abstract

The gene ( ) is involved in the auxin signaling pathway and stalk rot resistance in . In this study, we examined four transposon insertion lines (UFMu-03429, UFMu-00414, UFMu-08200, and AcDs-00676) targeting . Transcription of of the insertion lines either decreased or remained unchanged at the juvenile (V3) stage but increased significantly at the transition/adult (V6) stage. We also analyzed its upstream gene and downstream gene . Our results show that transposon insertions can induce stage-specific changes in gene expression that affect related biosynthetic pathways.

摘要

该基因( )参与生长素信号通路以及[植物名称]中的茎腐病抗性。在本研究中,我们检测了四个靶向[基因名称]的转座子插入系(UFMu - 03429、UFMu - 00414、UFMu - 08200和AcDs - 00676)。这些插入系的[基因名称]转录在幼苗期(V3)要么降低要么保持不变,但在过渡/成年期(V6)显著增加。我们还分析了其上游基因[上游基因名称]和下游基因[下游基因名称]。我们的结果表明,转座子插入可诱导基因表达的阶段特异性变化,从而影响相关生物合成途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5e7/12305366/681e1b678b2f/25789430-2025-micropub.biology.001640.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5e7/12305366/681e1b678b2f/25789430-2025-micropub.biology.001640.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5e7/12305366/681e1b678b2f/25789430-2025-micropub.biology.001640.jpg

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本文引用的文献

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Insertion of Transposable Elements in of Leading to LOSS of the Avirulent Function.转座因子插入到 导致无毒功能丧失。
Int J Mol Sci. 2023 Oct 24;24(21):15542. doi: 10.3390/ijms242115542.
2
Maize stalk rot caused by alters soil microbial composition and is directly inhibited by isolated from rhizosphere soil.由[具体病原菌名称未给出]引起的玉米茎腐病会改变土壤微生物组成,并受到从根际土壤中分离出的[具体抑制物未给出]的直接抑制。
Front Microbiol. 2022 Oct 20;13:986401. doi: 10.3389/fmicb.2022.986401. eCollection 2022.
3
The genomic ecosystem of transposable elements in maize.
玉米中转座元件的基因组生态系统。
PLoS Genet. 2021 Oct 14;17(10):e1009768. doi: 10.1371/journal.pgen.1009768. eCollection 2021 Oct.
4
The Auxin-Regulated Protein ZmAuxRP1 Coordinates the Balance between Root Growth and Stalk Rot Disease Resistance in Maize.生长素调节蛋白 ZmAuxRP1 协调玉米根系生长和茎腐病抗性之间的平衡。
Mol Plant. 2019 Mar 4;12(3):360-373. doi: 10.1016/j.molp.2018.10.005. Epub 2018 Oct 25.
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Transposable element insertions in fission yeast drive adaptation to environmental stress.转座子插入在裂殖酵母中驱动对环境胁迫的适应。
Genome Res. 2019 Jan;29(1):85-95. doi: 10.1101/gr.239699.118. Epub 2018 Dec 12.
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Ten things you should know about transposable elements.转座元件的十件必知事项
Genome Biol. 2018 Nov 19;19(1):199. doi: 10.1186/s13059-018-1577-z.
7
DNA transposons: nature and applications in genomics.DNA 转座子:在基因组学中的性质和应用。
Curr Genomics. 2010 Apr;11(2):115-28. doi: 10.2174/138920210790886871.
8
Benzoxazinoid biosynthesis, a model for evolution of secondary metabolic pathways in plants.苯并恶嗪类生物合成:植物次生代谢途径进化的模式。
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9
Sequence-indexed mutations in maize using the UniformMu transposon-tagging population.利用UniformMu转座子标签群体对玉米进行序列索引突变分析。
BMC Genomics. 2007 May 9;8:116. doi: 10.1186/1471-2164-8-116.
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Statistical analysis of real-time PCR data.实时荧光定量PCR数据的统计分析。
BMC Bioinformatics. 2006 Feb 22;7:85. doi: 10.1186/1471-2105-7-85.