Dong Qianqian, Liu Qingxiang, Goodwin Paul H, Deng Xiaoxu, Xu Wen, Xia Mingcong, Zhang Jie, Sun Runhong, Wu Chao, Wang Qi, Wu Kun, Yang Lirong
College of Life Sciences, Henan Agricultural University, Zhengzhou 450046, China.
Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Agricultural Microbiology Innovation Center, Zhengzhou 450002, China.
J Fungi (Basel). 2023 May 9;9(5):547. doi: 10.3390/jof9050547.
Fusarium crown rot (FCR) caused by is one of the most serious soil-borne diseases of wheat. Among 58 bacterial isolates from the rhizosphere soil of winter wheat seedlings, strain YB-1631 was found to have the highest in vitro antagonism to growth. LB cell-free culture filtrates inhibited mycelial growth and conidia germination of by 84.14% and 92.23%, respectively. The culture filtrate caused distortion and disruption of the cells. Using a face-to-face plate assay, volatile substances produced by YB-1631 inhibited growth by 68.16%. In the greenhouse, YB-1631 reduced the incidence of FCR on wheat seedlings by 84.02% and increased root and shoot fresh weights by 20.94% and 9.63%, respectively. YB-1631 was identified as based on the sequence and average nucleotide identity of the complete genome. The complete genome was 4,090,312 bp with 4357 genes and 45.92% GC content. In the genome, genes were identified for root colonization, including those for chemotaxis and biofilm production, genes for plant growth promotion, including those for phytohormones and nutrient assimilation, and genes for biocontrol activity, including those for siderophores, extracellular hydrolase, volatiles, nonribosomal peptides, polyketide antibiotics, and elicitors of induced systemic resistance. In vitro production of siderophore, β-1, 3-glucanase, amylase, protease, cellulase, phosphorus solubilization, and indole acetic acid were detected. YB-1631 appears to have significant potential in promoting wheat growth and controlling wheat FCR caused by .
由镰刀菌引起的小麦根腐病是小麦最严重的土传病害之一。在从小麦幼苗根际土壤分离出的58株细菌中，菌株YB - 1631对镰刀菌生长的体外拮抗作用最强。LB无细胞培养滤液分别抑制镰刀菌菌丝生长和分生孢子萌发84.14%和92.23%。该培养滤液导致细胞变形和破裂。通过面对面平板试验，YB - 1631产生的挥发性物质抑制镰刀菌生长68.16%。在温室中，YB - 1631使小麦幼苗根腐病发病率降低84.02%，并使根和地上部鲜重分别增加20.94%和9.63%。基于完整基因组的16S rRNA序列和平均核苷酸同一性，YB - 1631被鉴定为枯草芽孢杆菌。完整基因组为4,090,312 bp，有4357个基因，GC含量为45.92%。在基因组中，鉴定出了与根部定殖相关的基因，包括趋化性和生物膜形成相关基因；与植物生长促进相关的基因，包括植物激素和养分同化相关基因；以及与生物防治活性相关的基因，包括铁载体、细胞外水解酶、挥发性物质、非核糖体肽、聚酮类抗生素和诱导系统抗性激发子相关基因。检测到了铁载体、β - 1,3 - 葡聚糖酶、淀粉酶、蛋白酶、纤维素酶、解磷以及吲哚乙酸的体外产生。YB - 1631在促进小麦生长和防治由镰刀菌引起的小麦根腐病方面似乎具有巨大潜力。
