Department of Pharmaceutics, College of Pharmacy, King Khalid University, Asir-Abha 61421, Saudi Arabia.
Department of Clinical and Movement Neurosciences, UCL Institute of Neurology, Queen Square, London, United Kingdom.
Gene. 2023 Jan 30;851:147020. doi: 10.1016/j.gene.2022.147020. Epub 2022 Nov 4.
To assess, if the SARS-CoV-2 mutate in a similar pattern globally or has a specific pattern in any given population.
We report, the insertion of TTT at 11085, which adds an extra amino acid, F to the NSP6 at amino acid position 38. The highest occurrence of TTT insertion at 11,085 position was found in UK derived samples (65.97%). The second and third highest occurrence of the mutation were found in Australia (8.3%) and USA (4.16%) derived samples, respectively. Another important discovery of this study is the C27945T mutation, which translates into the termination of ORF-8 after 17 amino acids, reveals that the SARS-CoV-2 can replicate without the intact ORF-8 protein. We found that the 97% of C27945T mutation of global occurrence, occurred in Europe and the USA derived samples.
Two of the reported mutations (11085TTT insertion and C27945T nonsense), which seemed to reduce Type I interferon response are linked to specific geographical locations of the host and implicate region-specific mutations in the virus. The findings of this study signify that SARS-CoV-2 has the potential to adapt differently to different populations.
评估 SARS-CoV-2 是否在全球以相似模式发生突变，或者在特定人群中是否具有特定模式。
我们报告了在 11085 位插入 TTT，这在 NSP6 的氨基酸位置 38 处添加了一个额外的氨基酸 F。在英国来源的样本中发现了最高的 TTT 插入 11085 位（65.97%）。该突变的第二和第三高发生率分别在澳大利亚（8.3%）和美国（4.16%）来源的样本中发现。这项研究的另一个重要发现是 C27945T 突变，它导致 ORF-8 在 17 个氨基酸后终止，表明 SARS-CoV-2 可以在没有完整 ORF-8 蛋白的情况下复制。我们发现全球 97%的 C27945T 突变发生在欧洲和美国来源的样本中。
报告的两种突变（11085TTT 插入和 C27945T 无义突变）似乎降低了 I 型干扰素反应，与宿主的特定地理位置有关，并暗示病毒存在特定于区域的突变。这项研究的结果表明，SARS-CoV-2 有可能以不同的方式适应不同的人群。
