Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing, 102206, People's Republic of China.
College of Bioscience and Resources Environment, Beijing University of Agriculture, Beijing, 102206, People's Republic of China.
Genes Genomics. 2023 May;45(5):593-610. doi: 10.1007/s13258-022-01335-9. Epub 2022 Nov 8.
Hemerocallis fulva is an important ground cover plant widely used in urban greening. The analysis of the molecular mechanism underlying the drought response of H. fulva can lay a foundation for improving its adaptability and expanding its planting area.
To reveal the drought response mechanisms of H. fulva, identify candidate unigenes associated with drought response, and lay a foundation for further unigenes functional study and drought resistance improvement of H. fulva via genetic engineering.
RNA was isolated from H. fulva under different experimental conditions. De novo transcriptomic analysis of the samples was performed to screen drought response unigenes. The transcriptional changes of candidate drought response unigenes were verified by quantitative real-time PCR.
The differentially expressed unigenes and their functions were analyzed after H. fulva treated by PEG-simulated drought stress and rewatering. The candidate unigenes, associated with H. fulva drought response, were identified after transcriptome analysis. Then, the transcription level of drought response unigenes of H. fulva under different conditions was further verified. Abscisic acid, protein phosphorylation, sterol biosynthesis and ion transport were involved in drought response with quick restore in H. fulva. The response unigenes, involved in hormone (ABA, JA, CK and GA) signaling pathways, defense response, high light response, karrikin response and leaf shaping, can maintain at changed expression levels even after stress withdraw.
Hemerocallis fulva has unique drought response mechanism. Negative regulation mechanism may play more important roles in drought response of H. fulva. The analysis of candidate unigenes, associated with drought response, lays a foundation for further drought resistance improvement of H. fulva.
萱草是一种重要的地被植物，广泛应用于城市绿化。分析萱草抗旱响应的分子机制可以为提高其适应性和扩大种植面积奠定基础。
揭示萱草的抗旱响应机制，鉴定与抗旱响应相关的候选基因，为进一步研究萱草基因功能和通过遗传工程提高其抗旱性奠定基础。
从不同实验条件下的萱草中分离 RNA。对样品进行从头转录组分析，筛选抗旱响应基因。通过定量实时 PCR 验证候选干旱响应基因的转录变化。
PEG 模拟干旱胁迫和复水后，对萱草差异表达基因及其功能进行了分析。通过转录组分析鉴定了与萱草抗旱反应相关的候选基因。然后，进一步验证了萱草在不同条件下干旱反应基因的转录水平。ABA、蛋白磷酸化、甾醇生物合成和离子转运参与了萱草的抗旱反应，并在快速恢复后保持表达水平。参与激素（ABA、JA、CK 和 GA）信号通路、防御反应、高光反应、卡瑞卡丁反应和叶片形成的响应基因即使在胁迫解除后仍能保持表达水平的变化。
萱草具有独特的抗旱响应机制。负调控机制可能在萱草的抗旱响应中发挥更重要的作用。与抗旱反应相关的候选基因的分析为进一步提高萱草的抗旱性奠定了基础。
