Marshall Laboratory of Biomedical Engineering, International Cancer Center, Laboratory of Evolutionary Theranostics (LET), School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen, 518060, China.
Adv Mater. 2023 May;35(19):e2207951. doi: 10.1002/adma.202207951. Epub 2023 Mar 22.
Lactate oxidase (LOx) has attracted extensive interest in cancer diagnosis and therapy in recent years owing to its specific catalysis on l-lactate; its catalytic process consumes oxygen (O ) and generates a large amount of hydrogen peroxide (H O ) and pyruvate. Given high levels of lactate in tumor tissues and its tight correlation with tumor growth, metastasis, and recurrence, LOx-based biosensors including H O -based, O -based, pH-sensitive, and electrochemical have been designed for cancer diagnosis, and various LOx-based cancer therapy strategies including lactate-depletion-based metabolic cancer therapy/immunotherapy, hypoxia-activated chemotherapy, H O -based chemodynamic therapy, and multimodal synergistic cancer therapy have also been developed. In this review, the lactate-specific catalytic properties of LOx are introduced, and the recent advances on LOx-instructed cancer diagnostic or therapeutic platforms and corresponding biological applications are summarized. Additionally, the challenges and potential of LOx-based nanomedicines are highlighted.
近年来，由于乳过氧化物酶（LOx）对 L-乳酸具有特异性催化作用，其催化过程消耗氧气（O2）并生成大量的过氧化氢（H2O2）和丙酮酸，因此在癌症诊断和治疗方面引起了广泛关注。鉴于肿瘤组织中乳酸水平较高，且与肿瘤生长、转移和复发密切相关，已经设计了基于 LOx 的生物传感器，包括基于 H2O2 的、基于 O2 的、pH 敏感的和电化学的，用于癌症诊断，并且还开发了各种基于 LOx 的癌症治疗策略，包括基于乳酸耗竭的代谢癌症治疗/免疫疗法、缺氧激活化疗、基于 H2O2 的化学动力学治疗以及多模式协同癌症治疗。在本文中，介绍了 LOx 的乳酸特异性催化特性，并总结了 LOx 指导的癌症诊断或治疗平台及其相应生物学应用的最新进展。此外，还强调了基于 LOx 的纳米药物的挑战和潜力。
