Makgobole Mokgadi Ursula, Onwubu Stanley Chibuzor, Baruwa Abayomi, Mpofana Nomakhosi, Obiechefu Zodidi, Naidoo Deneshree, Khathi Andile, Mkhwanazi Blessing
School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Durban 4001, South Africa.
Department of Somatology, Faculty of Health Sciences, Durban University of Technology, Durban 4001, South Africa.
Mar Drugs. 2024 Dec 17;22(12):562. doi: 10.3390/md22120562.
Collagen, a critical biomaterial with wide applications in pharmaceuticals, cosmetics, and medical industries, can be sourced sustainably from fish scales. This study optimizes the extraction of collagen using Tris-Glycine buffer from fish scales via the Taguchi method. Various extraction parameters-buffer concentration, temperature, pH, and time-were evaluated to identify optimal conditions. Under optimal conditions (0.5 M of acetic acids, volume of acids of 100 mL, soaking time of 120 min, and Tris-Glycine buffer of 10 mL), the results demonstrate that temperature and buffer concentration significantly influence collagen yield, with a collagen purity of 17.14 ± 0.05 mg/g. value of 73.84% was obtained for the mathematical model). FTIR analysis confirmed the presence of characteristic collagen peaks at 1611 cm (amide I), 1523 cm (amide II), and 1300 cm (amide III), indicating the successful extraction of type I collagen. SDS-PAGE analysis revealed a protein banding pattern consistent with the molecular weight of collagen, and amino acid analysis shows high percentages of glycine (20.98%), proline (15.43%), and hydroxyproline (11.51%), implying fibrous collagen structures. The finding suggests that the Taguchi method offers an efficient and sustainable approach for collagen extraction, reducing waste from fish processing industries. Nevertheless, there is a need for further experimental validation to align with mathematical modeling on the optimized conditions.
胶原蛋白是一种关键的生物材料，在制药、化妆品和医疗行业有着广泛应用，它可以可持续地从鱼鳞中获取。本研究采用田口方法优化了用Tris-甘氨酸缓冲液从鱼鳞中提取胶原蛋白的工艺。评估了各种提取参数——缓冲液浓度、温度、pH值和时间——以确定最佳条件。在最佳条件下（0.5M乙酸、100mL酸体积、120分钟浸泡时间和10mL Tris-甘氨酸缓冲液），结果表明温度和缓冲液浓度对胶原蛋白产量有显著影响，胶原蛋白纯度为17.14±0.05mg/g。（数学模型的 值为73.84%）。傅里叶变换红外光谱（FTIR）分析证实，在1611cm（酰胺I）、1523cm（酰胺II）和1300cm（酰胺III）处存在特征性的胶原蛋白峰，表明成功提取了I型胶原蛋白。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析显示出与胶原蛋白分子量一致的蛋白条带模式，氨基酸分析表明甘氨酸（20.98%）、脯氨酸（15.43%）和羟脯氨酸（11.51%）的百分比很高，这意味着存在纤维状胶原蛋白结构。该研究结果表明，田口方法为胶原蛋白提取提供了一种高效且可持续的方法，减少了鱼类加工业的废弃物。然而，需要进一步的实验验证，使其与优化条件下的数学模型相符。
