Tziveleka Leto-Aikaterini, Ioannou Efstathia, Tsiourvas Dimitris, Berillis Panagiotis, Foufa Evangelia, Roussis Vassilios
Department of Pharmacognosy and Chemistry of Natural Products, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece.
Institute of Nanosciences and Nanotechnology, NCSR "Demokritos", Aghia Paraskevi 15310, Attiki, Greece.
Mar Drugs. 2017 May 29;15(6):152. doi: 10.3390/md15060152.
In search of alternative and safer sources of collagen for biomedical applications, the marine demosponges and , collected from the Aegean and the Ionian Seas, respectively, were comparatively studied for their insoluble collagen, intercellular collagen, and spongin-like collagen content. The isolated collagenous materials were morphologically, physicochemically, and biophysically characterized. Using scanning electron microscopy and transmission electron microscopy the fibrous morphology of the isolated collagens was confirmed, whereas the amino acid analysis, in conjunction with infrared spectroscopy studies, verified the characteristic for the collagen amino acid profile and its secondary structure. Furthermore, the isoelectric point and thermal behavior were determined by titration and differential scanning calorimetry, in combination with circular dichroism spectroscopic studies, respectively.
为了寻找用于生物医学应用的替代且更安全的胶原蛋白来源，分别从爱琴海和爱奥尼亚海采集的海洋海绵 和 ，对其不溶性胶原蛋白、细胞间胶原蛋白和海绵样胶原蛋白含量进行了比较研究。对分离出的胶原质材料进行了形态学、物理化学和生物物理学表征。使用扫描电子显微镜和透射电子显微镜证实了分离出的胶原蛋白的纤维形态，而氨基酸分析结合红外光谱研究则验证了胶原蛋白氨基酸谱及其二级结构的特征。此外，分别通过滴定和差示扫描量热法结合圆二色光谱研究测定了等电点和热行为。
