Tchegnitegni Billy Toussie, Ahmed Tehmina, Ngouafong Francis Tatong, Kamso Viviane Flore Kamlo, Teponno Rémy Bertrand, Lenta Bruno Ndjakou, Tapondjou Léon Azefack, Ali Arslan, Musharraf Syed Ghulam
Department of Chemistry, Faculty of Science, University of Dschang, Dschang P.O. Box 67, Cameroon.
HEJ, Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
Metabolites. 2022 Oct 25;12(11):1018. doi: 10.3390/metabo12111018.
This paper describes eight new triterpenoid saponins, including afzeliioside A (), four acetylated afzeliiosides as pairs of inseparable regioisomers, called afzeliiosides B/C () and D/E (), afzeliiosides F-H (), and a known impatiprin C (), which were isolated from the -BuOH fraction of the liana of . Their structures were established mainly by extensive spectroscopic analysis, including 1D and 2D NMR, HRFAB-MS, tandem ESI-MS/MS, and chemical methods, as well as a comparison of their spectral data with those of related compounds. All the isolates were screened for their cytotoxic activity against the CAL-27 oral squamous carcinoma cell line. Only compounds (EC = 36.0 μg/mL (32.7 μM)) exhibited moderate cytotoxic activity. This work presents the first chemical and biological investigation of and reports, for the first time, on the isolation of saponins in the genus .
本文描述了八种新的三萜皂苷，包括阿夫泽利苷A（）、四种乙酰化阿夫泽利苷，它们是不可分离的区域异构体对，称为阿夫泽利苷B/C（）和D/E（）、阿夫泽利苷F - H（）以及一种已知的因帕替林C（），这些化合物是从的藤本植物的正丁醇部分分离得到的。它们的结构主要通过广泛的光谱分析确定，包括一维和二维核磁共振、高分辨快原子轰击质谱、串联电喷雾质谱/质谱以及化学方法，还通过将它们的光谱数据与相关化合物的数据进行比较来确定。对所有分离物进行了针对CAL - 27口腔鳞状癌细胞系的细胞毒性活性筛选。只有化合物（半数有效浓度=36.0μg/mL（32.7μM））表现出中等细胞毒性活性。这项工作首次对进行了化学和生物学研究，并首次报道了该属中皂苷的分离。
