Department of Biotechnology, National Institute of Technology Warangal, Telangana, 506004, Kazipet, Hanamkonda, India.
Appl Biochem Biotechnol. 2023 Mar;195(3):1630-1643. doi: 10.1007/s12010-022-04217-8. Epub 2022 Nov 10.
Our current study reports the anti-bacterial activity of the gold nanoparticles (AuNps) synthesized by the green synthesis method using Vitex negundo plant leaves. The aqueous extract of Vitex negundo plant leaves are acting as the capping and stabilizing agent in the synthesis of AuNps. It is already evident from earlier studies that Vitex negundo is an abundant source of polyphenols, flavonoids, terpenoids, and many other biologically active compounds. The present study reveals the potential of biologically active compounds from the plant in the reduction reaction of chloroauric acid (HAuCl) into gold nanoparticles. The green synthesis method is adapted instead of the chemical method, which is toxic and more expensive. The gold nanoparticles subjected to characterization with the help of UV-visible spectroscopy, FTIR to determine functional groups, light scattering to estimate size and uniformity, scanning emission microscopy with EDX for accurate size and shape of AuNps, and X-ray diffraction to reveal the crystalline structure. The characteristics of AuNps formed are UV reading at 520 nm, FTIR showing the presence of phenols and alkenes, DLS, SEM, and XRD confirming the spherical shape with the size around 70-90 nm. The anti-bacterial activity of the gold nanoparticles is evaluated against four different species of bacteria, each two gram-positive and gram-negative. The gold nanoparticles formed by Vitex negundo show good anti-bacterial activity against Salmonella typhi and M. luteus bacteria with a zone of inhibition of 6 mm and 2 mm respectively. Furthermore, the cytotoxic activities of the gold nanoparticles are yet to be known to their full extent.
我们目前的研究报告了使用荩草植物叶片的绿色合成方法合成的金纳米粒子（AuNps）的抗菌活性。荩草植物叶片的水提物在 AuNps 的合成中充当了封端和稳定剂。早期的研究已经表明，荩草是多酚、类黄酮、萜类化合物和许多其他生物活性化合物的丰富来源。本研究揭示了植物中生物活性化合物在氯金酸（HAuCl）还原为金纳米粒子反应中的潜力。绿色合成方法代替了化学方法，因为化学方法具有毒性且成本更高。金纳米粒子在 UV-可见光谱、FTIR 确定官能团、光散射估计尺寸和均匀性、扫描电子显微镜与 EDX 用于准确的 AuNps 尺寸和形状、以及 X 射线衍射揭示晶体结构的帮助下进行了表征。形成的 AuNps 的特征是在 520nm 处的 UV 读数、FTIR 显示酚类和烯烃的存在、DLS、SEM 和 XRD 证实了球形形状，尺寸约为 70-90nm。金纳米粒子的抗菌活性针对四种不同的细菌进行了评估，每种两种革兰氏阳性菌和革兰氏阴性菌。荩草形成的金纳米粒子对伤寒沙门氏菌和 M. luteus 细菌表现出良好的抗菌活性，抑菌圈分别为 6mm 和 2mm。此外，金纳米粒子的细胞毒性活性还远未得到充分了解。
