Jonadet M, Meunier M T, Villie F, Bastide J P, Lamaison J L
J Pharmacol. 1986 Jan-Mar;17(1):21-7.
In vitro experiments were conducted on the inhibitory properties of extracts from Ribes nigrum L. and Alchemilla vulgaris L. (fractions A1 + A2, A1, A2) on activity of the proteolytic enzymes elastase, trypsin and alpha-chymotrypsin. Extracts from Ribes Nigrum L. and Alchemilla Vulgaris L. (Fraction A1) inhibited 50% of the activity of porcine pancreas elastase at concentrations of 0.56 mg/ml and 0.16 mg/ml, respectively, against a synthetic substrate. Inhibition was less effective on activity of trypsin and alpha-chymotrypsin. Marked in vivo angioprotective properties were shown by the compounds studied, except Fraction A2 of Alchemilla vulgaris L. which had no significant activity. The results suggest a possible role by these inhibitors in the protection of conjunctive and elastic tissues adversely affected by proteolytic enzymes. An additional advantage is their lack of toxicity.
对黑醋栗（Ribes nigrum L.）和普通羽衣草（Alchemilla vulgaris L.）提取物（组分A1 + A2、A1、A2）对蛋白水解酶弹性蛋白酶、胰蛋白酶和α-糜蛋白酶活性的抑制特性进行了体外实验。黑醋栗（Ribes Nigrum L.）和普通羽衣草（Alchemilla Vulgaris L.）提取物（组分A1）在浓度分别为0.56 mg/ml和0.16 mg/ml时，对合成底物抑制了50%的猪胰弹性蛋白酶活性。对胰蛋白酶和α-糜蛋白酶活性的抑制效果较差。除普通羽衣草（Alchemilla vulgaris L.）的组分A2无显著活性外，所研究的化合物均表现出明显的体内血管保护特性。结果表明这些抑制剂可能在保护受蛋白水解酶不利影响的结缔组织和弹性组织中发挥作用。另一个优点是它们没有毒性。
