Ito Zensho, Koido Shigeo, Kato Kumiko, Odamaki Toshitaka, Horiuchi Sankichi, Akasu Takafumi, Saruta Masayuki, Hata Taigo, Kumagai Yu, Fujioka Shuichi, Misawa Takeyuki, Xiao Jin-Zhong, Sato Nobuhiro, Ohkusa Toshifumi
Department of Internal Medicine, Division of Gastroenterology and Hepatology, The Jikei University School of Medicine, Kashiwa Hospital, Kashiwa City 277-8567, Chiba, Japan.
Institute of Clinical Medicine and Research, The Jikei University School of Medicine, Kashiwa City 277-8567, Chiba, Japan.
Cancers (Basel). 2022 Oct 31;14(21):5379. doi: 10.3390/cancers14215379.
Characteristic bile duct and gut microbiota have been identified in patients with chronic biliary tract disease. This study aimed to characterize the fecal and bile microbiota in biliary tract cancer (BTC) patients and their relationship. Patients with BTC ( = 30) and benign biliary disease (BBD) without cholangitis ( = 11) were included. Ten healthy, age-matched subjects were also recruited for fecal microbiota comparison. The fecal and bile duct microbiotas were analyzed by sequencing the 16S rRNA gene V3-V4 region. Live bacteria were obtained in the bile from three BTC patients by culture, and metagenomics-based identification was performed. Linear discriminant analysis effect size showed a higher abundance and a lower abundance, including that of and , in the BTC patients than in the other subjects. Ten of 17 operational taxonomic units (OTUs) assigned to in the bile were matched with the OTUs found in the BTC subject fecal samples. Furthermore, a bile-isolated strain possessed the carcinogenic bacterial colipolyketide synthase-encoding gene. was enriched in the BTC feces, and more than half of in the bile matched that in the feces at the OTU level. Our data suggests that fecal microbiota dysbiosis may contribute to BTC onset.
慢性胆道疾病患者已被鉴定出具有特征性的胆管和肠道微生物群。本研究旨在描述胆道癌（BTC）患者的粪便和胆汁微生物群特征及其关系。纳入了BTC患者（n = 30）和无胆管炎的良性胆道疾病（BBD）患者（n = 11）。还招募了10名年龄匹配的健康受试者进行粪便微生物群比较。通过对16S rRNA基因V3-V4区域进行测序来分析粪便和胆管微生物群。通过培养从3例BTC患者的胆汁中获得活菌，并进行基于宏基因组学的鉴定。线性判别分析效应大小显示，BTC患者中某些菌的丰度较高，而另一些菌的丰度较低，包括某菌和另一菌，与其他受试者相比。胆汁中分配到某菌的17个可操作分类单元（OTU）中有10个与BTC受试者粪便样本中发现的OTU相匹配。此外，一株胆汁分离菌株拥有致癌细菌的编码细菌聚酮合酶的基因。某菌在BTC粪便中富集，胆汁中该菌超过一半在OTU水平上与粪便中的相匹配。我们的数据表明，粪便微生物群失调可能导致BTC发病。
