Tsai Kai-Yen, Wu Deng-Chyang, Wu Wen-Jeng, Wang Jiunn-Wei, Juan Yung-Shun, Li Ching-Chia, Liu Chung-Jung, Lee Hsiang-Ying
Department of Post-Baccalaureate Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Biomedicines. 2022 Oct 23;10(11):2676. doi: 10.3390/biomedicines10112676.
Numerous microorganisms residing in the gastrointestinal and genitourinary tracts affect host health. We investigated stool and voided urine samples collected from patients with benign prostatic hyperplasia (BPH) or prostate cancer (PC) and a control group to explore the potential relationship between human microbiota and prostatic disease, and aimed to identify correlations and pathogenic taxonomic units. We studied microbial composition using 16S rRNA sequencing to identify operational taxonomic units (OTUs). Extracted genome was amplified and filtered sequences were used to classify OTUs based on their specific taxonomy. No statistically significant differences were observed in stool samples among the groups. However, urine samples indicated different microbiota compositions in different patient populations. The top five microbial genera that showed significant differences between the BPH and control groups were , , , , and . , , , , and were the genera with the largest proportion differences when comparing the PC and control groups. We discovered that the urine microbiota composition of the BPH and PC groups was distinct from that of the control group. Due to the impact of microbiota on prostatic disease, it is necessary to identify specific microbes for further research.
许多存在于胃肠道和泌尿生殖道的微生物会影响宿主健康。我们调查了从良性前列腺增生（BPH）或前列腺癌（PC）患者以及对照组收集的粪便和尿液样本，以探索人类微生物群与前列腺疾病之间的潜在关系，旨在确定相关性和致病分类单元。我们使用16S rRNA测序研究微生物组成，以识别操作分类单元（OTU）。提取的基因组被扩增，过滤后的序列用于根据其特定分类法对OTU进行分类。各组粪便样本中未观察到统计学上的显著差异。然而，尿液样本表明不同患者群体中的微生物群组成不同。在BPH组和对照组之间显示出显著差异的前五个微生物属是 、 、 、 和 。在比较PC组和对照组时， 、 、 、 和 是比例差异最大的属。我们发现BPH组和PC组的尿液微生物群组成与对照组不同。由于微生物群对前列腺疾病的影响，有必要识别特定微生物以进行进一步研究。
