Badr Eman Ae, El Sayed Ibrahim Eltantawy, Gabber Mohanad Kareem Razak, Ghobashy Eman Abd Elrehem, Al-Sehemi Abdullah G, Algarni Hamed, Elghobashy Yasser As
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Menoufia University, Shebeen El-Kom 32511, Egypt.
Department of Chemistry, Faculty of Science, Menoufia University, Shebeen El-Kom 32511, Egypt.
Biomedicines. 2022 Nov 1;10(11):2770. doi: 10.3390/biomedicines10112770.
Fighting external pathogens relies on the tight regulation of the gene expression of the immune system. Ferroptosis, which is a distinct form of programmed cell death driven by iron, is involved in the enhancement of follicular helper T cell function during infection. The regulation of RNA is a key step in final gene expression. The present study aimed to identify the expression level of antisense lncRNAs (A2M-AS1, DBH-AS1, FLVCR1-DT, and NCBP2AS2-1) and FLVCR1 in COVID-19 patients and its relation to the severity of the disease. COVID-19 patients as well as age and gender-matched healthy controls were enrolled in this study. The expression level of the antisense lncRNAs was measured by RT-PCR. Results revealed the decreased expression of A2M-AS1 and FLVCR1 in COVID-19 patients. Additionally, they showed the increased expression of DBH-AS1, FLVCR1-DT, and NCBP2AS2. Both FLVCR1-DT and NCBP2AS2 showed a positive correlation with interleukin-6 (IL-6). DBH-AS1 and FLVCR1-DT had a significant association with mortality, complications, and mechanical ventilation. A significant negative correlation was found between A2M-AS1 and NCBP2AS2-1 and between FLVCR1 and FLVCR1-DT. The study confirmed that the expression level of the antisense lncRNAs was deregulated in COVID-19 patients and correlated with the severity of COVID-19, and that it may have possible roles in the pathogenesis of this disease.
抵御外部病原体依赖于免疫系统基因表达的严格调控。铁死亡是一种由铁驱动的独特程序性细胞死亡形式，参与感染期间滤泡辅助性T细胞功能的增强。RNA的调控是最终基因表达的关键步骤。本研究旨在确定COVID-19患者中反义长链非编码RNA（A2M-AS1、DBH-AS1、FLVCR1-DT和NCBP2AS2-1）和FLVCR1的表达水平及其与疾病严重程度的关系。本研究纳入了COVID-19患者以及年龄和性别匹配的健康对照。通过逆转录聚合酶链反应（RT-PCR）测量反义长链非编码RNA的表达水平。结果显示，COVID-19患者中A2M-AS1和FLVCR1的表达降低。此外，他们还显示DBH-AS1、FLVCR1-DT和NCBP2AS2的表达增加。FLVCR1-DT和NCBP2AS2均与白细胞介素-6（IL-6）呈正相关。DBH-AS1和FLVCR1-DT与死亡率、并发症和机械通气有显著关联。在A2M-AS1与NCBP2AS2-1之间以及FLVCR1与FLVCR1-DT之间发现显著负相关。该研究证实，COVID-19患者中反义长链非编码RNA的表达水平失调，与COVID-19的严重程度相关，并且可能在该疾病的发病机制中发挥作用。
