Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
State Key Laboratory of Agrobiotechnology and Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Genes (Basel). 2022 Oct 22;13(11):1922. doi: 10.3390/genes13111922.
Plant growth-promoting rhizobacteria (PGPR) are widely used to improve soil nutrients and promote plant growth and health. However, the growth-promoting effect of a single PGPR on plants is limited. Here, we evaluated the effect of applying rhizobium 5038 (R5038) and two PGPR strains, MB35-5 (BA) and 3016 (PM), alone or in different combinations on the soil properties and rhizosphere bacterial community composition of soybean (). Additionally, metagenomic sequencing was performed to elucidate the profile of functional genes. Inoculation with compound microbial inoculant containing R5038 and BA (RB) significantly improved nodule nitrogenase activity and increased soil nitrogen content, and urease activity increased the abundance of the nitrogen cycle genes and and in the rhizosphere. In the treatment of inoculant-containing R5038 and PM (RP), significant changes were found for the abundance of and and the phosphorus cycle genes, and soil available phosphorus and phosphatase activity were increased. The RBP inoculants composed of three strains (R5038, BA and PM) significantly affected soybean biomass and the N and P contents of the rhizosphere. Compared with RB and RP, RBP consistently increased soybean nitrogen content, and dry weight. Overall, these results showed that several PGPR with different functions could be combined into composite bacterial inoculants, which coordinately modulate the rhizosphere microbial community structure and improve soybean growth.
植物促生根际细菌（PGPR）被广泛用于改善土壤养分，促进植物生长和健康。然而，单一 PGPR 对植物的促生效果有限。在这里，我们评估了单独或不同组合应用根瘤菌 5038（R5038）和两种 PGPR 菌株 MB35-5（BA）和 3016（PM）对大豆（）土壤性质和根际细菌群落组成的影响。此外，还进行了宏基因组测序以阐明功能基因的特征。接种含有 R5038 和 BA 的复合微生物菌剂（RB）显著提高了根瘤固氮酶活性，增加了土壤氮含量，脲酶活性增加了氮循环基因和在根际的丰度。在含有 R5038 和 PM 的接种剂（RP）处理中，发现和的丰度以及磷循环基因发生了显著变化，土壤有效磷和磷酸酶活性增加。由三种菌株（R5038、BA 和 PM）组成的 RBP 接种剂对大豆生物量和根际的 N 和 P 含量有显著影响。与 RB 和 RP 相比，RBP 一致地增加了大豆氮含量和干重。总体而言，这些结果表明，几种具有不同功能的 PGPR 可以组合成复合细菌接种剂，协调调节根际微生物群落结构，从而促进大豆生长。
