School of Animal Science and Technology, Hainan University, Haikou 570228, China.
Genes (Basel). 2022 Nov 8;13(11):2067. doi: 10.3390/genes13112067.
The efficiency of feed utilization determines the cost and economic benefits of pig production. In the present study, two pairs of full-sibling and two pairs of half-sibling female Landrace finishing pigs were selected, with each pair including individuals with different feed conversion rates, with liver and longissimus muscle tissue samples collected from each group for transcriptome analysis. A total of 561 differentially expressed genes (DEGs), among which 224 were up-regulated and 337 were down-regulated, were detected in the liver transcriptomes in the high-feed efficiency group compared to the low-feed efficiency group. The DEGs related to phosphorus and phosphate metabolism, arginine biosynthesis, chemical carcinogenesis, cytokine-cytokine receptor interaction, the biosynthesis of amino acids, and drug metabolism-cytochrome P450 in liver tissue were also associated with feed efficiency. In total, 215 DEGs were screened in the longissimus muscle tissue and were mainly related to disease and immune regulation, including complement and coagulation cascades, systemic lupus erythematosus, and prion diseases. The combination of gene expression and functional annotation results led to the identification of candidate feed efficiency-related biomarkers, such as , , , , , , and , members of cytochrome P450 family, and complement component family genes. Although the novel feed efficiency-related candidate genes need to be further evaluated by a larger sample size and functional studies, the present study identifies novel candidate biomarkers for the identification of functional SNPs underlying porcine feed efficiency.
饲料利用率的效率决定了养猪生产的成本和经济效益。在本研究中，选择了两对全同胞和两对半同胞的长白育肥母猪，每对都包括具有不同饲料转化率的个体，从每组收集肝脏和背最长肌组织样本进行转录组分析。与低饲料效率组相比，高饲料效率组的肝脏转录组中检测到 561 个差异表达基因（DEGs），其中 224 个上调，337 个下调。与磷和磷酸盐代谢、精氨酸生物合成、化学致癌作用、细胞因子-细胞因子受体相互作用、氨基酸生物合成以及药物代谢-细胞色素 P450 相关的 DEGs也与饲料效率有关。总共在背最长肌组织中筛选出 215 个 DEG，主要与疾病和免疫调节有关，包括补体和凝血级联、系统性红斑狼疮和朊病毒病。基因表达和功能注释结果的结合导致了候选饲料效率相关生物标志物的鉴定，如、、、、、、和、细胞色素 P450 家族和补体成分家族基因的成员。虽然需要通过更大的样本量和功能研究来进一步评估新的饲料效率相关候选基因，但本研究确定了用于鉴定猪饲料效率功能 SNP 的新的候选生物标志物。
