Panthi Khim Prasad, Gyawali Aashish, Pandeya Shiva, Sharma Bhusal Motee Lal, Neupane Bhanu Bhakta, Tiwari Arjun Prasad, Joshi Mahesh Kumar
Department of Chemistry, Trichandra Multiple Campus, Tribhuvan University, Kathmandu 44613, Nepal.
Central Department of Chemistry, Tribhuvan University, Kathmandu 44613, Nepal.
Membranes (Basel). 2022 Nov 2;12(11):1089. doi: 10.3390/membranes12111089.
Agricultural waste-based cellulose fibers have gained significant interest for a myriad of applications. a plant species, has been widely used for feeding animals, and the small branches' bark is used for making rope. Herein, we have extracted cellulose fibers from the bark of species via chemical treatments (including an alkaline treatment and bleaching). The gravimetric analysis revealed that the bark of contains cellulose (63.13%), hemicellulose (13.52%), lignin (15.13%), and wax (2.8%). Cellulose microfibre (CMF) has been synthesized from raw fibre via chemical treatment methods. The obtained cellulose fibers were crosslinked and employed as the matrix to encapsulate the bioactive plant extracts derived from the root of The microscopic images, XRD, FTIR, and antibacterial/antioxidant activity confirmed the encapsulation of natural extracts in the cellulose microfiber. The microscopic images revealed that the encapsulation of the natural extracts slightly increased the fiber's diameter. The XRD pattern showed that the extracted cellulose microfiber had an average crystalline size of 2.53 nm with a crystalline index of 30.4% compared to the crystalline size of 2.49 nm with a crystalline index of 27.99% for the plant extract incorporated membrane. The water uptake efficiency of the synthesized membrane increased up to 250%. The antimicrobial activity of the composite (the CMF-E membrane) was studied via the zone inhibition against gram-positive and gram-negative bacteria, and the result indicated high antibacterial activity. This work highlighted cellulose microfiber as an optimum substrate for antimicrobial scaffolds. In addition, this paper first reports the antimicrobial/antioxidant behavior of the composite membrane of the extract blended in the microfiber. This work revealed the potential applications of CMF-E membranes for wound healing scaffolds.
基于农业废弃物的纤维素纤维在众多应用中引起了广泛关注。某植物物种已被广泛用于饲养动物，其小树枝的树皮可用于制作绳索。在此，我们通过化学处理（包括碱处理和漂白）从该物种的树皮中提取了纤维素纤维。重量分析表明，该树皮含有纤维素（63.13%）、半纤维素（13.52%）、木质素（15.13%）和蜡（2.8%）。纤维素微纤维（CMF）已通过化学处理方法从原纤维合成。所得纤维素纤维经过交联，并用作基质来封装源自某植物根部的生物活性植物提取物。显微镜图像、XRD、FTIR以及抗菌/抗氧化活性证实了天然提取物被封装在纤维素微纤维中。显微镜图像显示，天然提取物的封装略微增加了纤维的直径。XRD图谱表明，提取的纤维素微纤维的平均晶体尺寸为2.53nm，结晶指数为30.4%，而掺入植物提取物的膜的晶体尺寸为2.49nm，结晶指数为27.99%。合成膜的吸水效率提高到了250%。通过对革兰氏阳性和革兰氏阴性细菌的抑菌圈研究了复合材料（CMF - E膜）的抗菌活性，结果表明其具有高抗菌活性。这项工作突出了纤维素微纤维作为抗菌支架的最佳基质。此外，本文首次报道了某提取物与某微纤维混合的复合膜的抗菌/抗氧化行为。这项工作揭示了CMF - E膜在伤口愈合支架方面的潜在应用。
