Centre for Biotechnology, School of Pharmaceutical Sciences, Siksha 'O' Anusandhan (Deemed to Be University), Bhubaneswar 751003, Odisha, India.
Molecules. 2022 Oct 27;27(21):7302. doi: 10.3390/molecules27217302.
(L.) Baill. ex Pierre of family Magnoliaceae, is a perennial tree with aromatic, ethnobotanical, and medicinal uses. The leaf is reported to have a myriad of therapeutic activities, however, there are limited reports available on the chemical composition of the leaf essential oil of . The present study explored the variation in the yield and chemical composition of leaf essential oil isolated from 52 accessions of . Through hydrodistillation, essential oil yield was obtained, varied in the range of 0.06 ± 0.003% and 0.31 ± 0.015% (/) on a fresh weight basis. GC-MS analysis identified a total of 65 phytoconstituents accounting for 90.23 to 98.90% of the total oil. Sesquiterpene hydrocarbons (52.83 to 65.63%) constituted the major fraction followed by sesquiterpene alcohols (14.71 to 22.45%). The essential oils were found to be rich in β-elemene (6.64 to 38.80%), γ-muurolene (4.63 to 22.50%), and β-caryophyllene (1.10 to 20.74%). Chemometrics analyses such as PCA, PLS-DA, sPLS-DA, and cluster analyses such as hierarchical clustering, i.e., dendrogram and partitional clustering, i.e., K-means classified the essential oils of populations into three different chemotypes: chemotype I (β-elemene), chemotype II (γ-muurolene) and chemotype III (β-caryophyllene). The chemical polymorphism analyzed in the studied populations would facilitate the selection of chemotypes with specific compounds. The chemotypes identified in the populations could be developed as promising bio-resources for conservation and pharmaceutical application and further improvement of the taxa.
（L.）Baill. ex Pierre 是木兰科的一种多年生树木，具有芳香、民族植物学和药用用途。据报道，该叶具有多种治疗活性，然而，关于其叶精油的化学成分的报道有限。本研究探讨了从 52 个 种的叶子中分离出的叶子精油的产量和化学成分的变化。通过水蒸馏获得精油产量，范围在 0.06±0.003%和 0.31±0.015%（/）之间，以新鲜重量为基础。GC-MS 分析鉴定了总共 65 种植物成分，占总油的 90.23%至 98.90%。倍半萜烃（52.83%至 65.63%）构成主要部分，其次是倍半萜醇（14.71%至 22.45%）。发现这些精油富含 β-榄烯（6.64%至 38.80%）、γ-愈创木烯（4.63%至 22.50%）和 β-石竹烯（1.10%至 20.74%）。化学计量学分析，如 PCA、PLS-DA、sPLS-DA 和聚类分析，如层次聚类，即树状图和分区聚类，即 K-均值，将 种群的精油分为三种不同的化学型：化学型 I（β-榄烯）、化学型 II（γ-愈创木烯）和化学型 III（β-石竹烯）。对研究种群进行的化学多态性分析将有助于选择具有特定化合物的化学型。在 种群中鉴定出的化学型可作为保护和药物应用的有前途的生物资源进一步发展，并进一步改进该分类单元。
