比较新鲜和干燥叶片水提物的组成、自由基清除能力和抑菌活性。

Comparison of Composition, Free-Radical-Scavenging Capacity, and Antibiosis of Fresh and Dry Leave Aqueous Extract from .

机构信息

Hainan Key Laboratory of Biology of Tropical Flowers and Trees Resources, Forestry Institute, Hainan University, Haikou 570228, China.

出版信息

Molecules. 2023 Aug 8;28(16):5935. doi: 10.3390/molecules28165935.

DOI:10.3390/molecules28165935

PMID:37630187

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10457956/

Abstract

Numerous plants of medicinal value grow on Hainan Island (China). Given the lack of knowledge on the phytochemical and pharmacological properties of Chun and Y. F. Wu (), the application of natural antioxidants and antimicrobials in the food industry has attracted increasing interest. This study aimed to compare the chemical composition, free-radical-scavenging capacity, and antibiosis of aqueous extracts of the fresh and dried leaves of The aqueous extract of the leaves of was obtained using steam distillation, and its chemical components were separated and identified via gas chromatography-mass spectrometry (GC-MS). The free-radical-scavenging capacity and antibiosis were determined. Further, 28 and 20 compounds were isolated from the fresh leaf aqueous extract of (MSFLAE) and dried leaf aqueous extract of (MSDLAE), respectively. The free-radical-scavenging capacity of MSFLAE and MSDLAE was determined by the 2,2-diphenyl-1 picrylhydrazyl (DPPH) method, which was 43.43% and 38.74%, respectively. The scavenging capacity of MSFLAE and MSDLAE determined by the 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate (ABTS)) method was 46.90% and 25.99%, respectively. The iron ion reduction capacity of MSFLAE and MSDLAE was determined by the ferric-reducing antioxidant power (FRAP) method as 94.7 and 62.9 μmol Fe⁺/L, respectively. This indicated that the two leaf aqueous extracts had a certain free-radical-scavenging capacity, and the capacity of MSFLAE was higher than that of MSDLAE. The antibiosis of the two leaf aqueous extracts on the three foodborne pathogenic bacteria was low, but the antimicrobial effects on Gram-positive bacteria were better than those on Gram-negative bacteria. The antibiosis of MSFLAE on and was greater than that of MSDLAE. Finally, MSFLAE and MSDLAE both had certain free-radical-scavenging capacities and antibiosis, confirming that the use of this plant in the research and development of natural antioxidants and antibacterial agents was reasonable. Plant aqueous extracts are an essential source of related phytochemistry and have immense pharmacological potential.

摘要

海南岛（中国）生长着大量具有药用价值的植物。由于缺乏对 Chun 和 Y. F. Wu（）植物化学成分和药理性质的了解，因此，天然抗氧化剂和抗菌剂在食品工业中的应用引起了越来越多的关注。本研究旨在比较新鲜和干燥的叶片的水提物的化学成分、自由基清除能力和抑菌作用。采用水蒸气蒸馏法提取叶片的水提物，通过气相色谱-质谱联用（GC-MS）对其化学成分进行分离和鉴定。测定了自由基清除能力和抑菌作用。此外，从新鲜叶水提物（MSFLAE）和干叶水提物（MSDLAE）中分别分离和鉴定了 28 种和 20 种化合物。通过 2,2-二苯基-1-苦基肼基（DPPH）法测定了 MSFLAE 和 MSDLAE 的自由基清除能力，分别为 43.43%和 38.74%。通过 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）法测定了 MSFLAE 和 MSDLAE 的清除能力，分别为 46.90%和 25.99%。通过铁还原抗氧化能力（FRAP）法测定了 MSFLAE 和 MSDLAE 的铁离子还原能力分别为 94.7 和 62.9 μmol Fe⁺/L，表明两种叶水提物均具有一定的自由基清除能力，且 MSFLAE 的清除能力高于 MSDLAE。两种叶水提物对三种食源性致病菌的抑菌作用较弱，但对革兰氏阳性菌的抑菌效果优于革兰氏阴性菌。MSFLAE 对和的抑菌作用大于 MSDLAE。最后，MSFLAE 和 MSDLAE 均具有一定的自由基清除能力和抑菌作用，证实了该植物在天然抗氧化剂和抗菌剂的研究与开发中的合理性。植物水提物是相关植物化学物质的重要来源，具有巨大的药理学潜力。