College of Tropical Crops, Hainan University, Haikou 570228, China.
Tropical Crops Genetic Resources Institute (CATAS), Danzhou 571737, China.
Molecules. 2022 Nov 2;27(21):7474. doi: 10.3390/molecules27217474.
The papaya industry is mainly impacted by viral diseases, especially papaya ringspot disease (PRSD) caused by papaya ringspot virus (PRSV). So far, research on the interaction between Chitosan, Lentinan and Ningnanmycin on PRSD has not been reported. This research studied the controlled and interactive effect of three biological agents, namely, Chitosan (C), Lentinan (L) and Ningnanmycin (N), on PRSV in papaya, individually and collectively. The changes in disease index, controlled effect, Peroxidase (POD), Polyphenol oxidase (PPO), Superoxide dismutase (SOD), growth and development of plants were observed at the seedling stage, in pots, and at the fruiting stage, in the field. The appearance and nutrient contents of fruits were measured during the fruit stage. The disease index of PRSV, at seedling and fruiting stages, was significantly lower for chitosan, lentinan and ningnanmycin and their interactive effect, compared to a control check treatment. The activity of the defense enzymes could be improved by the three kinds of biological agents and their interactive effect, especially lentinan and ningnanmycin. The chlorophyll content, plant height, stem diameter and fruit quality rose significantly under chitosan, lentinan and ningnanmycin treatments. The interaction of LN could inhibit PRSV disease at the seedling and fruiting stages of papaya, and promote the growth of plants and the quality of fruit at the fruit stage. Hence, this study provides the theoretical foundation for the biological control of papaya ringspot disease.
番木瓜产业主要受病毒病影响，特别是由番木瓜环斑病毒（PRSV）引起的番木瓜环斑病（PRSD）。迄今为止，尚未有关于壳聚糖、香菇多糖和宁南霉素对 PRSD 相互作用的研究报道。本研究研究了三种生物制剂，即壳聚糖（C）、香菇多糖（L）和宁南霉素（N），单独和联合对番木瓜 PRSV 的控制和相互作用。在苗期、盆栽和结果期田间观察了病情指数、控制效果、过氧化物酶（POD）、多酚氧化酶（PPO）、超氧化物歧化酶（SOD）、植物生长发育的变化，在果实期测量了果实的外观和营养成分。PRSV 的病情指数在苗期和结果期，壳聚糖、香菇多糖和宁南霉素及其互作对 PRSV 的病情指数明显低于对照处理。三种生物制剂及其互作可以提高防御酶的活性，特别是香菇多糖和宁南霉素。壳聚糖、香菇多糖和宁南霉素处理后，叶绿素含量、株高、茎径和果实品质显著提高。LN 的相互作用可以抑制番木瓜幼苗和果实期的 PRSV 病，促进植物生长和果实品质。因此，本研究为番木瓜环斑病的生物防治提供了理论基础。
