Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), E-08034 Barcelona, Spain.
Department of Cell Biology, Physiology & Immunology, Faculty of Biology, University of Barcelona, E-08028 Barcelona, Spain.
Nutrients. 2022 Nov 3;14(21):4656. doi: 10.3390/nu14214656.
The goal of this work is to explore if the changes induced by d-fagomine in the gut microbiota are compatible with its effect on body weight and inflammation markers in rats. Methods: Sprague Dawley rats were fed a standard diet supplemented with d-fagomine (or not, for comparison) for 6 months. The variables measured were body weight, plasma mediators of inflammation (hydroxyeicosatetraenoic acids, leukotriene B4, and IL-6), and the concentration of acetic acid in feces and plasma. The composition and diversities of microbiota in cecal content and feces were estimated using 16S rRNA metabarcoding and high-throughput sequencing. We found that after just 6 weeks of intake d-fagomine significantly reduced body weight gain, increased the plasma acetate concentration, and reduced the plasma concentration of the pro-inflammatory biomarkers' leukotriene B4, interleukin 6 and 12 hydroxyeicosatetraenoic acids. These changes were associated with a significantly increased prevalence of and feces and increased , , and while reducing , and in cecal content. In conclusion, d-fagomine induced changes in the composition and diversity of gut microbiota similar to those elicited by dietary fiber and compatible with its anti-inflammatory and body-weight-reducing effects.
本研究旨在探索地胆苦素诱导的肠道微生物群变化是否与其在大鼠体重和炎症标志物方面的作用相适应。方法：将 Sprague Dawley 大鼠用标准饮食喂养 6 个月，同时补充或不补充地胆苦素（作为对照）。测量的变量包括体重、血浆炎症介质（羟二十碳四烯酸、白三烯 B4 和 IL-6）以及粪便和血浆中乙酸的浓度。通过 16S rRNA 代谢组学和高通量测序来估计盲肠内容物和粪便中微生物群的组成和多样性。结果发现，仅摄入地胆苦素 6 周后，体重增加明显减少，血浆乙酸浓度增加，促炎生物标志物白三烯 B4、白细胞介素 6 和 12-羟二十碳四烯酸的血浆浓度降低。这些变化与粪便中 、 、 、 丰度的显著增加以及盲肠内容物中 、 、 丰度的降低有关。结论：地胆苦素诱导的肠道微生物群组成和多样性的变化与膳食纤维引起的变化相似，与其抗炎和减轻体重的作用相适应。
