发酵 pH 值对南海希瓦氏菌 QD 所产胞外多糖结构、流变性及抗氧化活性的影响
Effect of fermentation pH on the structure, rheological properties, and antioxidant activities of exopolysaccharides produced by Alteromonas australica QD.
机构信息
Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, China.
College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao, 266100, China.
出版信息
Glycoconj J. 2022 Dec;39(6):773-787. doi: 10.1007/s10719-022-10087-3. Epub 2022 Nov 11.
The pH value was essential for the growth and metabolism of microorganisms. Acidic pH exopolysaccharide (AC-EPS) and alkaline pH exopolysaccharide (AL-EPS) secreted by A. australica QD mediated by pH were studied in this paper. The total carbohydrate content and molecular weight of AC-EPS (79.59% ± 2.24% (w/w), 8.374 × 10 Da) and AL-EPS (82.48% ± 1.46% (w/w), 6.182 × 10 Da) were estimated and compared. In AC-EPS, mannose (3.78%) and galactose (3.24%) content was more, while the proportion of glucuronic acid was less in comparison to AL-EPS. The scanning electron microscopy revealed the structural differences among the AC-EPS and AL-EPS. Thermogravimetric analysis showed degradation temperatures of 272.8 °C and 244.9 °C for AC-EPS and AL-EPS, respectively. AC-EPS was found to exhibit better rheological properties and emulsifying capabilities, while AL-EPS had superior antioxidant activities. Overall, both AC-EPS and AL-EPS have the potential to be used as emulsifiers and biological antioxidants.
pH 值对微生物的生长和代谢至关重要。本文研究了 A. australica QD 分泌的酸性 pH 值胞外多糖（AC-EPS）和碱性 pH 值胞外多糖（AL-EPS）。测定并比较了 AC-EPS（79.59%±2.24%（w/w），8.374×10 Da）和 AL-EPS（82.48%±1.46%（w/w），6.182×10 Da）的总糖含量和分子量。在 AC-EPS 中，甘露糖（3.78%）和半乳糖（3.24%）的含量较高，而与 AL-EPS 相比，葡萄糖醛酸的比例较低。扫描电子显微镜揭示了 AC-EPS 和 AL-EPS 之间的结构差异。热重分析表明，AC-EPS 和 AL-EPS 的降解温度分别为 272.8°C 和 244.9°C。发现 AC-EPS 具有更好的流变性能和乳化能力，而 AL-EPS 具有更好的抗氧化活性。总的来说，AC-EPS 和 AL-EPS 都有可能用作乳化剂和生物抗氧化剂。
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