Martinez-Gonzalez Marta, Fidalgo-Pereira Rita C, Torres Orlanda, Silva Filipe, Henriques Bruno, Özcan Mutlu, Souza Júlio C M
University Institute of Health Sciences (IUCS), CESPU, 4585-116, Gandra PRD, Portugal.
Faculty of Dental Medicine, Universidade Católica Portuguesa, 3504-505, Viseu, Portugal.
Odontology. 2023 Apr;111(2):310-327. doi: 10.1007/s10266-022-00758-w. Epub 2022 Nov 12.
The main aim of this study was to perform an integrative review on the toxic effects of resin-matrix cements and their products in contact with fibroblasts or mesenchymal cells. A bibliographic search was performed on PubMed using the following search terms: "cytotoxicity" AND "fibroblast" OR "epithelial" OR "mesenchymal" AND "polymerization" OR "degree of conversion" OR "methacrylate" OR "monomer" AND "resin cement" OR "resin-based cement". The initial search in the available database yielded a total of 277 articles of which 21 articles were included in this review. A decrease in the viability of mouse fibroblasts ranged between 13 and 15% that was recorded for different resin-matrix cements after light curing exposure for 20 s. The viability of human fibroblasts was recorded at 83.11% after light curing for 20 s that increased up to 90.9% after light curing exposure for 40 s. Most of the studies linked the highest toxicity levels when the cells were in contact with Bis-GMA followed by UDMA, TEGDMA and HEMA. Resin-matrix cements cause a cytotoxic reaction when in contact with fibroblasts or mesenchymal cells due to the release of monomers from the polymeric matrix. The amount of monomers released from the resin matrix and their cytotoxicity depends on the polymerization parameters.
本研究的主要目的是对树脂基质水门汀及其产品与成纤维细胞或间充质细胞接触时的毒性作用进行综合综述。在PubMed上使用以下检索词进行文献检索：“细胞毒性”与“成纤维细胞”或“上皮细胞”或“间充质细胞”以及“聚合”或“转化率”或“甲基丙烯酸酯”或“单体”以及“树脂水门汀”或“树脂基水门汀”。在现有数据库中的初步检索共得到277篇文章，其中21篇文章被纳入本综述。不同树脂基质水门汀在光照固化20秒后，小鼠成纤维细胞的活力下降幅度在13%至15%之间。人成纤维细胞在光照固化20秒后的活力记录为83.11%，在光照固化40秒后增加到90.9%。大多数研究表明，当细胞与双酚A甲基丙烯酸缩水甘油酯（Bis-GMA）接触时毒性水平最高，其次是二甲基丙烯酸脲烷（UDMA）、三乙二醇二甲基丙烯酸酯（TEGDMA）和甲基丙烯酸羟乙酯（HEMA）。树脂基质水门汀与成纤维细胞或间充质细胞接触时会引起细胞毒性反应，这是由于聚合物基质中单体的释放。从树脂基质中释放的单体量及其细胞毒性取决于聚合参数。
