Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Hangzhou, China.
Department of Stomatology, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, Fujian, China.
J Mech Behav Biomed Mater. 2023 Jan;137:105567. doi: 10.1016/j.jmbbm.2022.105567. Epub 2022 Nov 10.
The study investigated the effects of an orthodontic primer containing amorphous fluorinated calcium phosphate (AFCP) nanoparticles on enamel white spot lesions (WSLs).
The AFCP nanoparticles were prepared and incorporated into Transbond XT Primer. Thirty-two human enamel slices were highly polished and randomly divided into four groups: no part covered (control), half covered with a primer containing 0 wt%, 25 wt%, and 35 wt% AFCP. Subsequently, samples were challenged by a modified pH-cycling and characterized by color measurement, micro-computed tomography, and scanning electron microscope (SEM). The bonding properties of the primers containing AFCP were assessed using shear bond strength test, and the mouse fibroblasts (L929) were employed to evaluate the cytotoxicity.
When the enamel was challenged by pH cycling, 25 wt% and 35 wt% AFCP groups exhibited less color change (ΔE) and less mineral loss than the control and 0 wt% AFCP groups. The SEM images showed that the original microstructural integrity and mineral deposition rate of the enamel surface were better in the 25 wt% and 35 wt% AFCP groups. In particular, the 35 wt% AFCP group exhibited the best performance after 3 weeks of pH cycling. The shear bond strength and cell viability revealed no significant difference among the tested groups (P > 0.05).
Using the primer containing 35 wt% AFCP might be a promising strategy for preventing the occurrence and development of WSLs during orthodontic treatment.
本研究旨在探讨含有非晶态氟化钙磷（AFCP）纳米颗粒的正畸底漆对釉质白斑（WSL）的影响。
制备 AFCP 纳米颗粒并将其掺入 Transbond XT 底漆中。将 32 个人类釉质切片高度抛光并随机分为四组：无部分覆盖（对照）、用含有 0wt%、25wt%和 35wt%AFCP 的底漆覆盖一半。随后，通过改良的 pH 循环对样品进行挑战，并通过颜色测量、微计算机断层扫描和扫描电子显微镜（SEM）进行表征。使用剪切结合强度测试评估含有 AFCP 的底漆的结合性能，并使用小鼠成纤维细胞（L929）评估细胞毒性。
当釉质受到 pH 循环的挑战时，25wt%和 35wt%AFCP 组的颜色变化（ΔE）和矿物质损失均小于对照组和 0wt%AFCP 组。SEM 图像显示，在 25wt%和 35wt%AFCP 组中，釉质表面的原始微观结构完整性和矿物质沉积率更好。特别是在 pH 循环 3 周后，35wt%AFCP 组表现出最佳性能。剪切结合强度和细胞活力在测试组之间没有显著差异（P>0.05）。
使用含有 35wt%AFCP 的底漆可能是预防正畸治疗中 WSL 发生和发展的一种有前途的策略。
