Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 300044, Taiwan.
Microbiology and Immunology Department and Immunology Program, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Sci Rep. 2022 Nov 16;12(1):19661. doi: 10.1038/s41598-022-24344-w.
Hydrogen peroxide (HO) is an important molecule in biological and environmental systems. In living systems, HO plays essential functions in physical signaling pathways, cell growth, differentiation, and proliferation. Plasmonic nanostructures have attracted significant research attention in the fields of catalysis, imaging, and sensing applications because of their unique properties. Owing to the difference in the reduction potential, silver nanostructures have been proposed for the detection of HO. In this work, we demonstrate the Au@Ag nanocubes for the label- and enzyme-free detection of HO. Seed-mediated synthesis method was employed to realize the Au@Ag nanocubes with high uniformity. The Au@Ag nanocubes were demonstrated to exhibit the ability to monitor the HO at concentration levels lower than 200 µM with r = 0.904 of the calibration curve and the limit of detection (LOD) of 1.11 µM. In the relatively narrow range of the HO at concentration levels lower than 40 µM, the LOD was calculated to be 0.60 µM with r = 0.941 of the calibration curve of the HO sensor. This facile fabrication strategy of the Au@Ag nanocubes would provide inspiring insights for the label- and enzyme-free detection of HO
过氧化氢（HO）是生物和环境系统中的重要分子。在生命系统中，HO 在物理信号通路、细胞生长、分化和增殖中发挥着重要作用。由于其独特的性质，等离子体纳米结构在催化、成像和传感应用等领域引起了广泛的研究关注。由于还原电位的差异，银纳米结构已被提议用于检测 HO。在这项工作中，我们展示了 Au@Ag 纳米立方体用于无标记和无酶检测 HO。采用种子介导合成方法实现了具有高均匀性的 Au@Ag 纳米立方体。Au@Ag 纳米立方体被证明具有在低于 200 μM 的浓度水平下监测 HO 的能力，校准曲线的 r 值为 0.904，检测限（LOD）为 1.11 μM。在浓度水平低于 40 μM 的相对较窄的 HO 范围内，HO 传感器的校准曲线的 r 值为 0.941，LOD 计算为 0.60 μM。这种简单的 Au@Ag 纳米立方体的制造策略将为无标记和无酶检测 HO 提供新的思路。
