Hemmati Mahdi, Selakjan Amir Hossein Q, Ghasemi Forough
Department of Nanotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran.
Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran.
Sci Rep. 2025 Feb 3;15(1):4050. doi: 10.1038/s41598-025-88342-4.
As a naturally occurring reducing and oxidizing agent, hydrogen peroxide (HO) has a role in several biotic and abiotic processes. Hence, the onsite, precise, and rapid determination of HO is crucial. Herein, we propose a method for colorimetric detection of HO on the basis of hindered formation of gold/silver core/shell nanoparticles. We used ascorbic acid (AA) as the electron donor to reduce silver ions (Ag) to be shelled around gold nanoparticles and iron(III) edta as an accelerator reactant. Upon reduction of Ag, owing to the formation of core/shell nanoparticles, the color of the system changes from pink to yellow/orange in the spherical nanoparticles and from pink to purple/blue/green/yellow/orange in the nanorods. The nanorods distinguished color in a rainbow manner for higher concentrations of HO, and spherical nanoparticles were critical in the sensitive detection of lower concentrations of HO. HO scavenges AA electrons and therefore inhibits core/shell formation and, consequently, restrains the system's spectral shift and color change. This characteristic was exploited to measure different concentrations of HO. Under well-optimized conditions, various concentrations of HO ranging from 1.0 to 50 µΜ have shown an acceptable linear relationship with different colors and, with a limit of detection (LOD) of 230 nM. Furthermore, various real samples were examined to confirm the practicality of our developed probe.
作为一种天然存在的还原剂和氧化剂，过氧化氢（H₂O₂）在多个生物和非生物过程中发挥作用。因此，现场、精确且快速地测定H₂O₂至关重要。在此，我们提出一种基于阻碍金/银核壳纳米颗粒形成的比色法检测H₂O₂的方法。我们使用抗坏血酸（AA）作为电子供体，将银离子（Ag⁺）还原以包覆在金纳米颗粒周围，并使用铁（III）乙二胺四乙酸作为促进剂反应物。在Ag⁺还原后，由于核壳纳米颗粒的形成，体系颜色在球形纳米颗粒中从粉红色变为黄色/橙色，在纳米棒中从粉红色变为紫色/蓝色/绿色/黄色/橙色。纳米棒对于较高浓度的H₂O₂以彩虹方式区分颜色，而球形纳米颗粒对于较低浓度H₂O₂的灵敏检测至关重要。H₂O₂清除AA的电子，因此抑制核壳形成，进而抑制体系的光谱位移和颜色变化。利用这一特性来测量不同浓度的H₂O₂。在优化良好的条件下，1.0至50 μΜ的各种浓度H₂O₂与不同颜色呈现出可接受的线性关系，检测限（LOD）为230 nM。此外，对各种实际样品进行了检测，以证实我们所开发探针的实用性。
