Du Jun, Shi Yan, Zhou Changming, Guo Lianying, Hu Ruiming, Huang Cheng, Hu Guoliang, Gao Xiaona, Guo Xiaoquan
Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.
School of Computer and Information Engineering, Jiangxi Agricultural University, Nanchang, China.
Front Vet Sci. 2022 Oct 28;9:1052553. doi: 10.3389/fvets.2022.1052553. eCollection 2022.
In this study, we investigated the therapeutic effect and mechanism of action of vitamin C on chronic heat stress (CHS)-induced liver oxidative damage and inflammation in laying hens. The thermoneutral control group (TN group) was kept at a constant temperature of 22 ± 1°C, while the chronic heat stress group (CHS group) and the vitamin C supplemented group (HSV group) were exposed to heat stress (HS) (36 ± 1°C, 8 h/d). The TN and HS groups were fed the basic diet at will, and the HSV group was supplemented with 300 mg/kg of vitamin C on top of the basic diet. The experimental results showed a significant improvement in body weight and feed intake in the HSV group compared to the HS group. A significantly lower pH and higher and PCO levels were observed in the HSV group compared to the CHS group. As laying hens were supplemented with vitamin C, serum malondialdehyde (MDA) level was declined, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were increased, and total antioxidant capacity (T-AOC) was increased. Further, CHS induced an increase in the expression of inflammation-related genes and a decrease in the expression of antioxidant-related genes. In contrast, the addition of vitamin C reversed the effects of CHS, resulting in an increase in the expression of antioxidant-related genes and a decrease in the expression of inflammation-related genes. In conclusion, vitamin C can effectively alleviate CHS-induced acid-base imbalance in body fluids of laying hens and the oxidative damage and inflammatory response caused to the liver. Therefore, vitamin C can be used clinically as an effective drug to alleviate chronic heat stress in laying hens. This experiment provides clinical evidence and theoretical basis for the use of vitamin C as an effective drug to alleviate chronic heat stress in laying hens.
在本研究中，我们探究了维生素C对蛋鸡慢性热应激（CHS）诱导的肝脏氧化损伤和炎症的治疗效果及作用机制。热中性对照组（TN组）保持在22±1°C的恒定温度下，而慢性热应激组（CHS组）和维生素C补充组（HSV组）则暴露于热应激（HS）（36±1°C，每天8小时）环境中。TN组和HS组随意采食基础日粮，HSV组在基础日粮之上补充300 mg/kg的维生素C。实验结果表明，与HS组相比，HSV组的体重和采食量有显著改善。与CHS组相比，HSV组的pH值显著降低， 和PCO水平升高。随着蛋鸡补充维生素C，血清丙二醛（MDA）水平下降，超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性增加，总抗氧化能力（T-AOC）增强。此外，CHS导致炎症相关基因表达增加，抗氧化相关基因表达减少。相反，添加维生素C逆转了CHS的影响，导致抗氧化相关基因表达增加，炎症相关基因表达减少。总之，维生素C可以有效缓解CHS诱导的蛋鸡体液酸碱失衡以及对肝脏造成的氧化损伤和炎症反应。因此，维生素C可在临床上用作缓解蛋鸡慢性热应激的有效药物。本实验为维生素C作为缓解蛋鸡慢性热应激的有效药物的应用提供了临床证据和理论依据。
