原发性干燥综合征中粪便和血浆代谢相关诊断标志物的鉴定
Identification of diagnostic markers related to fecal and plasma metabolism in primary Sjögren's syndrome.
作者信息
Han Jianxing, Zeng Aiming, Hou Ziqi, Xu Yanan, Zhao Hua, Wang Bei, Guan Wenzhao, An Ying, Liang Shufen, Ma Yufeng
机构信息
Department of Stomatology, The Second Hospital of Shanxi Medical University Taiyuan 030001, Shanxi, P. R. China.
Department of Laboratory, The Second Hospital of Shanxi Medical University Taiyuan 030001, Shanxi, P. R. China.
出版信息
Am J Transl Res. 2022 Oct 15;14(10):7378-7390. eCollection 2022.
BACKGROUND
Accurate diagnostic techniques for patients with primary Sjögren's syndrome (pSS) are needed. This study aimed to investigate new biomarkers related to fecal and plasma metabolism from pSS patients.
METHODS
The feces and plasma of 21 pSS patients and 18 controls admitted to the Second Hospital of Shanxi Medical University were collected for analysis. Metabolites in feces and plasma were quantified using liquid chromatography-mass spectrometry. The metabolic pathway alterations caused by pSS were studied and the expression of metabolites in the intersecting pathway was analyzed in the feces and plasma of pSS patients. Metabolites that showed the same alterations in feces and plasma in pSS patients were considered as diagnostic markers and receiver operating characteristic curves were generated to analyze the sensitivity of these markers in diagnosing pSS.
RESULTS
There were 114 and 92 upregulated metabolites and 54 and 125 downregulated metabolites in the feces and plasma of pSS patients, respectively. These metabolites were enriched in 8 pathways for feces and 12 pathways for plasma. Arginine biosynthesis, Linoleic acid metabolism, Tyrosine metabolism, Taurine and hypotaurine metabolism were pathways enriched by metabolites in both samples. Twelves metabolites were enriched in the above four pathways, while only 9,10-12,13-Diepoxyoctadecanoate, Tyramine, 9-OxoODE and 2-Hydroxyethanesulfonate showed the same trend. The candidate diagnostic markers were all predictive, with better diagnostic sensitivity in plasma samples.
CONCLUSIONS
9,10-12,13-Diepoxyoctadecanoate, Tyramine, 9-OxoODE, 2-Hydroxyethanesulfonate were metabolism-related diagnostic markers for pSS feces and plasma.
背景
原发性干燥综合征（pSS）患者需要准确的诊断技术。本研究旨在探究与pSS患者粪便和血浆代谢相关的新生物标志物。
方法
收集山西医科大学第二医院收治的21例pSS患者和18例对照的粪便和血浆进行分析。采用液相色谱 - 质谱法定量粪便和血浆中的代谢物。研究pSS引起的代谢途径改变，并分析pSS患者粪便和血浆中相交途径中代谢物的表达。将pSS患者粪便和血浆中表现出相同改变的代谢物视为诊断标志物，并生成受试者工作特征曲线以分析这些标志物在诊断pSS中的敏感性。
结果
pSS患者粪便中有114种上调代谢物和54种下调代谢物，血浆中有92种上调代谢物和125种下调代谢物。这些代谢物在粪便的8条途径和血浆的12条途径中富集。精氨酸生物合成、亚油酸代谢、酪氨酸代谢、牛磺酸和低牛磺酸代谢是两个样本中代谢物富集的途径。12种代谢物在上述四条途径中富集，而只有9,10 - 12,13 - 二环氧十八烷酸、酪胺、9 - 氧代十八碳二烯酸和2 - 羟基乙磺酸盐表现出相同趋势。候选诊断标志物均具有预测性，在血浆样本中具有更好的诊断敏感性。
结论
9,10 - 12,13 - 二环氧十八烷酸、酪胺、9 - 氧代十八碳二烯酸、2 - 羟基乙磺酸盐是pSS粪便和血浆的代谢相关诊断标志物。
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