Chen Huan, Yan Han, Xiu Yan, Jiang Linlin, Zhang Jianlong, Chen Guozhong, Yu Xin, Zhu Hongwei, Zhao Xiaoyu, Li Youzhi, Tang Wenli, Zhang Xingxiao
College of Life Science, Ludong University, Yantai, Shandong, China.
Shandong Breeding Environmental Control Engineering Laboratory, Ludong University, Yantai, China.
Front Vet Sci. 2022 Nov 7;9:1019005. doi: 10.3389/fvets.2022.1019005. eCollection 2022.
The bacteria contained in air aerosols from poultry houses are closely connected to animal health and production. This study aimed to investigate the seasonal factors on microbial aerosol concentration, particle size and bacterial spectrum composition inside a closed-cage broiler house. Then, 16S rDNA sequencing technology was applied to analyze the characteristics of bacterial abundance and diversity. The results indicated that the concentration of bacterial aerosol in the broiler house varied significantly in different seasons, with a concentration range of 5.87-15.77 × 10 CFU/m, and the highest and lowest concentrations in the summer and winter, respectively. Microbiological analysis showed that the proportion of Gram-negative bacteria in autumn was significantly higher than that in summer ( < 0.05). In addition, the floral structure of potential pathogenic bacterial genera also differed by season. were identified in the bacterial aerosols. Importantly, the relative abundance of in spring and autumn was much higher. In contrast, the relative abundance of in spring and autumn was lower than that in summer and winter. Altogether, results revealed the effects of seasonal factors on the diversity and abundance of bacteria and the distribution characteristics of major opportunistic pathogens in the air of closed-cage broiler houses. These results will provide important information for exploring the potential risk of aerosols from poultry houses all four seasons.
禽舍空气气溶胶中的细菌与动物健康和生产密切相关。本研究旨在调查封闭式肉鸡舍内微生物气溶胶浓度、粒径和细菌谱组成的季节因素。然后，应用16S rDNA测序技术分析细菌丰度和多样性特征。结果表明，肉鸡舍内细菌气溶胶浓度在不同季节差异显著，浓度范围为5.87-15.77×10 CFU/m，夏季和冬季浓度分别最高和最低。微生物分析表明，秋季革兰氏阴性菌比例显著高于夏季（<0.05）。此外，潜在致病细菌属的菌群结构也因季节而异。在细菌气溶胶中被鉴定出来。重要的是，春季和秋季的相对丰度要高得多。相比之下，春季和秋季的相对丰度低于夏季和冬季。总之，结果揭示了季节因素对封闭式肉鸡舍空气中细菌多样性和丰度以及主要机会性病原菌分布特征的影响。这些结果将为探索四季禽舍气溶胶的潜在风险提供重要信息。
