Yan Han, Chen Huan, Jiang Linlin, Zhang Jianlong, Chen Guozhong, Yu Xin, Zhu Hongwei, Zhao Xiaoyu, Li Youzhi, Tang Wenli, Zhang Xingxiao, Jiang Nihong
College of Life Science, Ludong University, Yantai, Shandong, China.
Shandong Breeding Environmental Control Engineering Laboratory, Ludong University, Yantai, Shandong, China.
J Air Waste Manag Assoc. 2023 May;73(5):417-427. doi: 10.1080/10962247.2023.2193162.
Microbial aerosols in intensive broiler houses whose species and concentrations are closely related to human health are ubiquitous. Based on 16S rRNA gene sequencing, the aim of this study was to investigate the spatial distribution and diversity of bacterial aerosols in the air of broiler houses. Significant spatial variations in airborne bacterial concentrations were observed inside the poultry farmhouse. The results indicated that bacteria in the air samples could be grouped into a total of 1,674 OTUs. Alpha diversity analysis showed that the diversity of the microbial community at the entry of the broiler house was higher than that at the middle or the rear ( < 0.01). The Sankey diagram illustrated species dynamic changes in , , and among the different locations. From the aspect of LEfSe (LDA Effect Size) analysis, we discovered that the abundance of was significantly higher in the entry than in the rear and middle. This study shows the spatial distribution of the entire bacterial community in intensive broiler houses, which offers a new perspective for studying airborne total bacteria in those environments. The bacteria contained in air aerosols from poultry houses are closely connected to animal health and production. This study aimed to investigate the spatial distribution and diversity of bacterial aerosols in the air of broiler houses. The results observed that bacterial aerosol concentrations in the examined broilers house varied greatly at different positions, and a significantly higher exposure to bacterial aerosol was observed at the middle than at the other positions (p < 0.05). The alpha diversity analysis showed that the diversity of the microbial community at the entry of the broiler house was higher than that at the middle or the rear (P<0.01). Sankey diagram illustrated species dynamic changes of and among the different locations. The microbial communities in genus level in the samples of entry and rear were closer, while the species diversity of middle and rear samples in chicken house was highly similar (P>0.05). Altogether, results revealed that the effects of spatial factors on the diversity and abundance of bacteria in the air of closed-cage broiler houses, which poses a potential threat to the health of animals and workers in those environments.
集约化肉鸡舍中微生物气溶胶普遍存在，其种类和浓度与人类健康密切相关。基于16S rRNA基因测序，本研究旨在调查肉鸡舍空气中细菌气溶胶的空间分布和多样性。在家禽舍内观察到空气中细菌浓度存在显著的空间差异。结果表明，空气样本中的细菌总共可分为1674个操作分类单元（OTU）。α多样性分析表明，肉鸡舍入口处微生物群落的多样性高于中间或后部（P<0.01）。桑基图展示了不同位置之间物种的动态变化。从线性判别分析效应大小（LEfSe）分析方面来看，我们发现入口处的丰度显著高于后部和中间位置。本研究展示了集约化肉鸡舍中整个细菌群落的空间分布，为研究这些环境中的空气总细菌提供了新的视角。家禽舍空气气溶胶中所含细菌与动物健康和生产密切相关。本研究旨在调查肉鸡舍空气中细菌气溶胶的空间分布和多样性。结果观察到，在所检查的肉鸡舍中，细菌气溶胶浓度在不同位置差异很大，并且观察到中间位置的细菌气溶胶暴露显著高于其他位置（P<0.05）。α多样性分析表明，肉鸡舍入口处微生物群落的多样性高于中间或后部（P<0.01）。桑基图展示了不同位置之间物种的动态变化。入口和后部样本在属水平上的微生物群落更接近，而鸡舍中间和后部样本的物种多样性高度相似（P>0.05）。总之，结果揭示了空间因素对封闭式笼养肉鸡舍空气中细菌多样性和丰度的影响，这对这些环境中的动物和工人健康构成潜在威胁。
