Department of Animal Resources Science, Dankook University, Cheonan 31116, Republic of Korea.
Division of Food and Animal Science, Chungbuk National University, Cheongju 28644, Republic of Korea.
J Microbiol Biotechnol. 2021 Dec 28;31(12):1701-1708. doi: 10.4014/jmb.2109.09038.
Food safety is the most important global health issue due to foodborne pathogens after consumption of contaminated food. Foodborne bacteria such as , , , spp., , spp., and are leading causes of the majority of foodborne illnesses and deaths. These foodborne pathogens often come from the livestock feces, thus, we analyzed fecal microbial communities of three different livestock species to investigate the prevalence of foodborne pathogens in livestock feces using metagenomics analysis. Our data showed that alpha diversities of microbial communities were different according to livestock species. The microbial diversity of cattle feces was higher than that of chicken or pig feces. Moreover, microbial communities were significantly different among these three livestock species (cattle, chicken, and pig). At the genus level, and were found in all livestock feces, with chicken feces having higher relative abundances of and than cattle and pig feces. Genera , , and were detected in cattle feces. Chicken samples contained , , and with low relative abundance. Other genera such as , , , , , , and known to be opportunistic pathogens were also detected in cattle, chicken, and pig feces. Results of this study might be useful for controlling the spread of foodborne pathogens in farm environments known to provide natural sources of these microorganisms.
食品安全是最重要的全球性健康问题，因为食用受污染的食物后会导致食源性病原体。食源性细菌，如 、 、 、 、 、 和 ，是大多数食源性疾病和死亡的主要原因。这些食源性病原体通常来自牲畜粪便，因此，我们分析了三种不同牲畜物种的粪便微生物群落，通过宏基因组分析来调查食源性病原体在牲畜粪便中的流行情况。我们的数据表明，根据牲畜种类的不同，微生物群落的 alpha 多样性也不同。牛的粪便微生物多样性高于鸡或猪的粪便。此外，这三种牲畜（牛、鸡和猪）的微生物群落存在显著差异。在属水平上，所有牲畜粪便中都发现了 和 ，鸡粪便中 和 的相对丰度高于牛和猪粪便。在牛粪便中检测到属 、 和 。鸡样本中含有相对低丰度的 、 和 。其他已知的机会性病原体属，如 、 、 、 、 、 和 ，也在牛、鸡和猪粪便中检测到。本研究的结果可能有助于控制已知是这些微生物天然来源的农场环境中食源性病原体的传播。
