Liu Jianfeng, Hu Xianwen, He Hongli, Zhang Xingzheng, Guo Jinhua, Bai Jing, Cheng Yunqing
Jilin Provincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping, Jilin, China.
College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.
Front Microbiol. 2022 Nov 2;13:1025771. doi: 10.3389/fmicb.2022.1025771. eCollection 2022.
Soybean Sclerotinia stem rot caused by is a common disease in soybean, and effective biological control is urgently needed. We have previously confirmed that can effectively antagonize in a plate competition experiment and a soybean seedling inoculation experiment. In this study, the mechanisms underlying plant death caused by and soybean resistance to induced by were evaluated. The stems of potted soybean seedlings were inoculated with (Gm-Ss), (Gm-Ba), and their combination (Gm-Ba-Ss), using scratch treatments as a control, followed by dual RNA sequencing and bioinformatics analyses. Global gene expression levels in the Gm-Ss treatment were much lower than those in the Gm-Ba, Gm-Ba-Ss, and Gm groups, suggesting that strongly inhibited global gene expression in soybean. In a pairwise comparison of Gm-Ss vs. Gm, 19983 differentially expressed genes (DEGs) were identified. Down-regulated DEGs were involved in various KEGG pathways, including ko01110 (biosynthesis of secondary metabolites), ko01100 (metabolic pathways), ko01120 (microbial metabolism in diverse environments), ko00500 (starch and sucrose metabolism), and ko04075 (plant hormone signal transmission), suggesting that inoculation had a serious negative effect on soybean metabolism. In Gm-Ba vs. Gm, 13091 DEGs were identified, and these DEGs were significantly enriched in ko03010 (ribosome) and ko03008 (ribosome biogenesis in eucaryotes). Our results suggest that increases the expression of genes encoding the ribosomal subunit, promotes cell wall biogenesis, and induces systemic resistance. strongly inhibited metabolism in soybean, inhibited the synthesis of the cytoskeleton, and induced the up-regulation of programmed death and senescence-related genes an ethylene signal transduction pathway. These results improve our understanding of induced plant death and soybean resistance to induced by and may contribute to the improvement of strategies to avoid yield losses.
由[病原菌名称未给出]引起的大豆菌核病是大豆中的常见病害，迫切需要有效的生物防治方法。我们之前在平板竞争实验和大豆幼苗接种实验中证实了[拮抗菌名称未给出]能够有效拮抗[病原菌名称未给出]。在本研究中，评估了由[病原菌名称未给出]引起的植物死亡机制以及[拮抗菌名称未给出]诱导的大豆对[病原菌名称未给出]的抗性机制。对盆栽大豆幼苗的茎进行接种处理，分别接种[病原菌名称未给出]（Gm-Ss）、[拮抗菌名称未给出]（Gm-Ba）及其组合（Gm-Ba-Ss），以划破处理作为对照，随后进行双RNA测序和生物信息学分析。Gm-Ss处理组中的全局基因表达水平远低于Gm-Ba、Gm-Ba-Ss和Gm组，这表明[病原菌名称未给出]强烈抑制了大豆中的全局基因表达。在Gm-Ss与Gm的成对比较中，鉴定出19983个差异表达基因（DEGs）。下调的DEGs涉及多种KEGG途径，包括ko01110（次生代谢物的生物合成）、ko01100（代谢途径）、ko01120（不同环境中的微生物代谢）、ko00500（淀粉和蔗糖代谢）以及ko04075（植物激素信号转导），这表明接种[病原菌名称未给出]对大豆代谢有严重的负面影响。在Gm-Ba与Gm的比较中，鉴定出13091个DEGs，这些DEGs在ko03010（核糖体）和ko03008（真核生物中的核糖体生物发生）中显著富集。我们的结果表明，[拮抗菌名称未给出]增加了编码核糖体亚基的基因的表达，促进了细胞壁生物合成，并诱导了系统抗性。[病原菌名称未给出]强烈抑制了大豆中的代谢，抑制了细胞骨架的合成，并诱导了程序性死亡和衰老相关基因（乙烯信号转导途径中的基因）的上调。这些结果增进了我们对诱导植物死亡以及[拮抗菌名称未给出]诱导的大豆对[病原菌名称未给出]的抗性的理解，并可能有助于改进避免产量损失的策略。
