Lin Shudai, Xian Mingjian, Ren Tuanhui, Mo Guodong, Zhang Li, Zhang Xiquan
College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong, China.
Department of Animal Genetics Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, China.
Front Physiol. 2022 Nov 3;13:1033075. doi: 10.3389/fphys.2022.1033075. eCollection 2022.
The birth weight of chickens does not significantly affect the weight at slaughter, while the different growth rate after birth was one of the important reasons for the difference in slaughter weight. Also, the increase in chickens' postnatal skeletal muscle weight is the main cause of the slaughter weight gain, but which genes are involved in this biological process is still unclear. In this study, by integrating four transcriptome datasets containing chicken muscles at different developmental times or different chicken tissues in public databases, a total of nine candidate genes that may be related to postnatal muscle development in chickens were obtained, including , , , , , , , , and . One of these candidate genes is , whose 42 bp insertion/deletion (indel) mutation significantly correlated with multiple carcass traits in the F2 resource population from Xinghua chickens crossing with White Recessive Rock (WRR) chickens, including live weight, carcass weight, half eviscerated weight, eviscerated weight, breast meat weight, wing weight, leg muscle shear force, and breast muscle shear force. Also, there was a very significant difference between different genotypes of the 42 bp indel mutation in these trains. Further experiments showed that was highly expressed in chicken skeletal muscle, and its overexpression could promote the proliferation and inhibit the differentiation of chicken myoblasts by regulating and expression. Our findings demonstrated that the 42 bp indel may be one of the molecular markers of chicken weight-related traits.
鸡的出生体重对屠宰体重没有显著影响，而出生后不同的生长速度是导致屠宰体重差异的重要原因之一。此外，鸡出生后骨骼肌重量的增加是屠宰体重增加的主要原因，但参与这一生物学过程的基因仍不清楚。在本研究中，通过整合公共数据库中包含不同发育时期鸡肌肉或不同鸡组织的四个转录组数据集，共获得了九个可能与鸡出生后肌肉发育相关的候选基因，包括 、 、 、 、 、 、 、 和 。其中一个候选基因是 ，其42 bp插入/缺失（indel）突变与杏花鸡与隐性白洛克鸡（WRR）杂交的F2资源群体中的多个胴体性状显著相关，包括活重、胴体重、半净膛重、全净膛重、胸肉重、翅重、腿肌剪切力和胸肌剪切力。此外，在这些性状中，42 bp indel突变的不同基因型之间存在非常显著的差异。进一步的实验表明， 在鸡骨骼肌中高表达，其过表达可通过调节 和 的表达来促进鸡成肌细胞的增殖并抑制其分化。我们的研究结果表明，42 bp indel可能是鸡体重相关性状的分子标记之一。
