Zhang Xiaoxiao, Liu Xiang, Zhou Minghui, Hu Yonghong, Yuan Junhui
College of Landscape Architecture and Arts, Northwest A&F University, Yangling, Shaanxi, China.
Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai, China.
Front Plant Sci. 2022 Nov 3;13:1030584. doi: 10.3389/fpls.2022.1030584. eCollection 2022.
, a widely cultivated tree peony species in China, is a resourceful plant with medicinal, ornamental and oil value. However, fleshy roots lead to a low tolerance to waterlogging in . In this study, roots were sequenced using a hybrid approach combining single-molecule real-time and next-generation sequencing platforms to understand the molecular mechanism underlying the response to this sequentially waterlogging stress, the normal growth, waterlogging treatment (WT), and waterlogging recovery treatment (WRT). Our results indicated that the strategy of , in response to WT, was a hypoxic resting syndrome, wherein the glycolysis and fermentation processes were accelerated to maintain energy levels and the tricarboxylic acid cycle was inhibited. enhanced waterlogging tolerance by reducing the uptake of nitrate and water from the soil. Moreover, transcription factors, such as AP2/EREBP, WRKY, MYB, and NAC, played essential roles in response to WT and WRT. They were all induced in response to the WT condition, while the decreasing expression levels were observed under the WRT condition. Our results contribute to understanding the defense mechanisms against waterlogging stress in .
作为中国广泛种植的牡丹品种，是一种具有药用、观赏和油用价值的资源丰富的植物。然而，肉质根导致其对涝渍的耐受性较低。在本研究中，采用单分子实时测序和新一代测序平台相结合的混合方法对根系进行测序，以了解其对这种顺序性涝渍胁迫、正常生长、涝渍处理（WT）和涝渍恢复处理（WRT）响应的分子机制。我们的结果表明，对WT的响应策略是一种缺氧静止综合征，其中糖酵解和发酵过程加速以维持能量水平，三羧酸循环受到抑制。通过减少从土壤中吸收硝酸盐和水分来提高耐涝性。此外，转录因子，如AP2/EREBP、WRKY、MYB和NAC，在对WT和WRT的响应中起重要作用。它们在WT条件下均被诱导，而在WRT条件下观察到表达水平下降。我们的结果有助于理解其对涝渍胁迫的防御机制。
