Centre for Biomedical Engineering, University of Surrey, Guildford, UK.
DNAe, London, UK.
Electrophoresis. 2023 Jun;44(11-12):947-955. doi: 10.1002/elps.202200234. Epub 2022 Nov 21.
Microfluidic devices for dielectrophoretic cell separation are typically designed and constructed using microfabrication methods in a clean room, requiring time and expense. In this paper, we describe a novel alternative approach to microfluidic device manufacture, using chips cut from conductor-insulator laminates using a cutter plotter. This allows the manufacture of microchannel devices with micron-scale electrodes along every wall. Fabrication uses a conventional desktop cutter plotter, and requires no chemicals, masks or clean-room access; functional fluidic devices can be designed and constructed within a couple of hours at negligible cost. As an example, we demonstrate the construction of a continuous dielectrophoretic cell separator capable of enriching yeast cells to 80% purity at 10 000 cells/s.
微流控装置用于介电泳细胞分离，通常使用微制造方法在洁净室中设计和构建，需要时间和费用。在本文中，我们描述了一种使用切绘机从导体-绝缘体层压板上切割芯片来制造微流控器件的新方法。这种方法允许在每个壁上制造具有微米级电极的微通道器件。制造使用传统的桌面切绘机，不需要化学物质、掩模或洁净室通道；功能流体设备可以在几个小时内以可忽略的成本设计和构建。作为一个例子，我们展示了一个连续介电泳细胞分离器的构建，该分离器能够以 10,000 个细胞/秒的速度将酵母细胞富集到 80%的纯度。
