Zhang Yu, Chen Wenchan, Shao Wenyong, Tan Shishan, Shi Dongya, Ma Hongyu, Chen Changjun
The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Department of Plant Pathology, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China.
The Key Laboratory of Pesticide, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
J Fungi (Basel). 2022 Nov 11;8(11):1189. doi: 10.3390/jof8111189.
Smi1 is a protein required for cell cycle progression, morphogenesis, stress response and life span of . FaSmi1 was identified as a Smi1 homolog in a wheat scab pathogenic fungus strain 2021. The deletion of FaSmi1 leads to defects in mycelial growth, asexual reproduction, and virulence. The FaSmi1 deletion mutant also exhibited increased sensitivity to osmotic stresses generated by NaCl and KCl, but increased tolerance to oxidative stresses and cell wall integrity inhibitors. All of these defects were restored by genetic complementation of the mutant with the whole parental gene. Interestingly, the antioxidant system-associated genes exhibit a lower expression level and the mycotoxins' DON content was decreased in the FaSmi1 deletion mutant compared with the parental strain 2021. These results indicate that FaSmi1 plays a critical role in the vegetative development, asexual reproduction, DON production and virulence of .
Smi1是细胞周期进程、形态发生、应激反应和[具体物种]寿命所必需的一种蛋白质。FaSmi1在小麦赤霉病致病真菌菌株2021中被鉴定为Smi1的同源物。FaSmi1的缺失导致菌丝生长、无性繁殖和毒力出现缺陷。FaSmi1缺失突变体对NaCl和KCl产生的渗透胁迫也表现出更高的敏感性，但对氧化胁迫和细胞壁完整性抑制剂的耐受性增强。通过用整个亲本基因对突变体进行遗传互补，所有这些缺陷都得到了恢复。有趣的是，与亲本菌株2021相比，FaSmi1缺失突变体中与抗氧化系统相关的基因表达水平较低，霉菌毒素DON的含量也有所降低。这些结果表明，FaSmi1在[具体物种]的营养发育、无性繁殖、DON产生和毒力中起关键作用。
