School of Physics and Electronic Information, Shangrao Normal University, Shangrao 334001, China.
School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China.
Molecules. 2022 Nov 17;27(22):7973. doi: 10.3390/molecules27227973.
In this study, a novel three-dimensional hollow mesoporous bioactive glass nanofiber scaffold has been synthesized with a template-assisted sol-gel method using bacterial cellulose (BC) as a template and nonionic triblock copolymer (P123) as a pore-directing agent, ethyl orthosilicate (TEOS), calcium nitrate tetrahydrate (CN), and triethyl phosphate (TEP) as glass precursors. Scanning and transmission electron microscopies, X-ray diffraction, nitrogen adsorption-desorption, and nuclear magnetic resonance method were applied to characterize the morphology, crystal structure, and chemical structure of the mesoporous bioactive glass nanofiber scaffold. Furthermore, the in vitro bioactivity and biocompatibility were also explored. The obtained scaffold depicted nanofiber-like morphology and interconnected three-dimensional network structure that replicated the BC template. The scaffold showed a large specific surface area (230.0 cm g) and pore volume (0.2 m g). More importantly, the scaffold exhibited excellent apatite-forming ability and cellular biocompatibility. We believe that the hollow mesoporous bioactive glass nanofiber scaffold has great potential application in bone tissue regeneration.
在这项研究中，我们采用模板辅助溶胶-凝胶法，以细菌纤维素（BC）为模板，非离子型三嵌段共聚物（P123）为孔导向剂，正硅酸乙酯（TEOS）、硝酸钙四水合物（CN）和三乙磷酸酯（TEP）为玻璃前驱体，成功合成了一种新型的三维中空介孔生物活性玻璃纳米纤维支架。我们采用扫描电子显微镜、透射电子显微镜、X 射线衍射、氮气吸附-脱附和核磁共振等方法对介孔生物活性玻璃纳米纤维支架的形貌、晶体结构和化学结构进行了表征。此外，我们还探索了其体外生物活性和生物相容性。结果表明，所获得的支架呈现出纳米纤维状形貌和相互连接的三维网络结构，复制了 BC 模板。该支架具有较大的比表面积（230.0 cm g）和孔体积（0.2 m g）。更重要的是，支架表现出优异的磷灰石形成能力和细胞生物相容性。我们相信，中空介孔生物活性玻璃纳米纤维支架在骨组织再生方面具有巨大的潜在应用价值。
