Yun Hui-suk, Kim Seung-eon, Hyun Yong-taek, Heo Su-jin, Shin Jung-wook
Center for Future Technology, Korea Institute of Materials Science, Changwon, Gyeongnam, South Korea 641-83.
J Biomed Mater Res B Appl Biomater. 2008 Nov;87(2):374-80. doi: 10.1002/jbm.b.31114.
Hierarchically 2D/3D mesoporous-macroporous bioactive glasses (MMBG) with good molding capabilities and compressive modulus were synthesized by sol-gel method and evaporation-induced self-assembly process in the presence of both nonionic triblock copolymers, EO(70)PO(20)EO(70) (P123) or EO(100)PO(65)EO(100) (F127), templates and methyl cellulose template. P123 or F127 acts as both a template, inducing the formation of mesopore, and an effective dispersant of MC, which produces macropores. In vitro bioactivity studies were carried out in simulated body fluid and showed superior bone-forming bioactivities of hierarchical MMBG. Human osteoblastlike cells, MG63, were seeded on MMBG and were determined using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5,-diphenyl-tetrazolium bromide] assay to confirm biocompatibilities of MMBG.
通过溶胶 - 凝胶法以及在非离子三嵌段共聚物EO(70)PO(20)EO(70)（P123）或EO(100)PO(65)EO(100)（F127）模板和甲基纤维素模板存在下的蒸发诱导自组装过程，合成了具有良好成型能力和压缩模量的分级二维/三维介孔 - 大孔生物活性玻璃（MMBG）。P123或F127既作为诱导介孔形成的模板，又作为产生大孔的甲基纤维素的有效分散剂。在模拟体液中进行了体外生物活性研究，结果表明分级MMBG具有优异的骨形成生物活性。将人成骨样细胞MG63接种在MMBG上，并使用MTT [3 - (4,5 - 二甲基噻唑 - 2 - 基)-2,5 - 二苯基溴化四氮唑] 测定法来确定MMBG的生物相容性。
