Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu 300, Taiwan.
Department of Life Science, Fu Jen Catholic University, New Taipei City 24205, Taiwan.
Molecules. 2022 Nov 17;27(22):7982. doi: 10.3390/molecules27227982.
In an effort to explore bioactive anti-inflammatory compounds from natural resources from all over Taiwan and various ecological environments, an active strain of was collected at Taitung County in Taiwan, prepared from soil origin. A bioassay-guided fractionation of the BuOH extract of a culture broth of a new strain of the actinomycete led to the isolation of five previously undescribed compounds: acrocarpunicains A-F (-). The structures were elucidated by 1D and 2D Nuclear Magnetic Resonance (NMR) spectroscopy and mass spectrometry. Furthermore, the isolated compounds were subjected to in vitro testing to evaluate their anti-inflammatory activity. Of these isolates, acrocarpunicains A (), B (), C () and F () showed NO inhibitory activity with IC values of 9.36 ± 0.25, 10.11 ± 0.47, 5.15 ± 0.18, and 27.17 ± 1.87 μM, stronger than the positive control, quercetin (IC = 35.95 ± 2.34 μM). To the best of our knowledge, this is the first report on azaphilone and phenanthrene-type metabolites from the genus .
为了从台湾各地和各种生态环境的天然资源中探索具有生物活性的抗炎化合物，从台湾台东采集了一株放线菌的活性菌株，该菌株源自土壤。一种新型放线菌培养物的 BuOH 提取物经生物活性导向分离，得到了五个以前未描述的化合物：acrocarpunicains A-F (-)。通过 1D 和 2D 核磁共振（NMR）光谱和质谱解析了这些结构。此外，对分离得到的化合物进行了体外测试，以评估其抗炎活性。在这些分离物中，acrocarpunicains A ()、B ()、C () 和 F () 对 NO 没有抑制活性，IC 值分别为 9.36 ± 0.25、10.11 ± 0.47、5.15 ± 0.18 和 27.17 ± 1.87 μM，比阳性对照槲皮素（IC = 35.95 ± 2.34 μM）更强。据我们所知，这是首次从属中报道氮杂菲酮和菲类代谢物。
