Department of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, Poland.
Students' Scientific Association, Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Bialystok, ul. Mickiewicza 2a, 15-230 Białystok, Poland.
Molecules. 2022 Nov 19;27(22):8034. doi: 10.3390/molecules27228034.
L. is from the genus (Primulaceae), and the understanding of its phytochemical and pharmacological properties is limited. In this study, the use of chromatographic techniques led to the isolation of a further eleven compounds, including three new flavonoids: 2',5-dihydroxyflavone 2'---glucopyranoside, 5,6-dihydroxyflavone 6--(6"--glucopyranosyl)--glucopyranoside (hottonioside A), and 4',5,7-trihydroxyflavone 7--(2"---glucuronide)--glucopyranoside. Their structures were determined using extensive 1D and 2D NMR data and mass spectrometry (HRMS). The qualitative assessment of the chemical composition of the investigated extracts and fractions was performed using the LC-HRMS technique. Furthermore, the antioxidant potential of extracts, fractions, and compounds and their ability to inhibit acetylcholinesterase were also evaluated. Thus, we may conclude that the observed biological effects are the result of the presence of many biologically active compounds, of which dibenzoylmethane is the most active. Therefore, is a source of substances with desirable properties in the prevention and treatment of neurodegenerative diseases.
L. 来自 （报春花科）属，其植物化学和药理学性质的了解有限。在这项研究中，使用色谱技术导致分离出另外 11 种化合物，包括三种新的类黄酮：2'，5-二羟基黄酮 2'---葡萄糖苷、5,6-二羟基黄酮 6--（6"--葡萄糖基）--葡萄糖苷（霍顿尼奥斯苷 A）和 4'，5,7-三羟基黄酮 7--（2"---葡萄糖醛酸苷）--葡萄糖苷。它们的结构通过广泛的 1D 和 2D NMR 数据和质谱（HRMS）确定。使用 LC-HRMS 技术对研究提取物和馏分的化学成分进行定性评估。此外，还评估了提取物、馏分和化合物的抗氧化潜力及其抑制乙酰胆碱酯酶的能力。因此，我们可以得出结论，观察到的生物学效应是存在许多具有生物活性的化合物的结果，其中二苯甲酰甲烷是最活跃的。因此， 是预防和治疗神经退行性疾病的具有理想特性的物质的来源。
