计算机辅助的寡核苷酸分析为大肠杆菌中RNA聚合酶-启动子识别提供了一个模型序列。
A computer aided oligonucleotide analysis provides a model sequence for RNA polymerase-promoter recognition in E.coli.
作者信息
Scherer G E, Walkinshaw M D, Arnott S
出版信息
Nucleic Acids Res. 1978 Oct;5(10):3759-73. doi: 10.1093/nar/5.10.3759.
Abstract
A novel computer procedure has been used to search for homology among 17 known procaryotic promoter sequences. A model sequence, :formula: (see text), is compatible with the properties of all known promoter and operator mutations, predicts base positions for the initiation of RNA synthesis coinciding with those determined experimentally, is compatible with current models for the regulation of transcription, suggests that RNA polymerase could recognize the DNA double helix firstly in the B conformation then in the A.
摘要
一种新颖的计算机程序已被用于搜索17个已知原核生物启动子序列之间的同源性。一个模型序列（见正文）与所有已知启动子和操纵基因突变的特性相符，预测RNA合成起始的碱基位置与实验确定的位置一致，与当前转录调控模型相符，表明RNA聚合酶首先可以识别B构象的DNA双螺旋，然后识别A构象的DNA双螺旋。
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